Rapid transcriptome and proteome profiling of a non-model marine invertebrate, Bugula neritina

Vorschaubild nicht verfügbar
Dateien
Zu diesem Dokument gibt es keine Dateien.
Datum
2010
Autor:innen
Wang, Hao
Zhang, Huoming
Wong, Yue Him
Ravasi, Timothy
B. Bajic, Vladimir
Qian, Pei-Yuan
Herausgeber:innen
Kontakt
ISSN der Zeitschrift
eISSN
item.preview.dc.identifier.isbn
Bibliografische Daten
Verlag
Schriftenreihe
URI (zitierfähiger Link)
DOI (zitierfähiger Link)
ArXiv-ID
Internationale Patentnummer
Link zur Lizenz
oops
EU-Projektnummer
Projekt
Open Access-Veröffentlichung
Sammlungen
Gesperrt bis
Titel in einer weiteren Sprache
Forschungsvorhaben
Organisationseinheiten
Zeitschriftenheft
Publikationstyp
Zeitschriftenartikel
Publikationsstatus
Published
Erschienen in
PROTEOMICS ; 10 (2010), 16. - S. 2972-2981. - Wiley. - ISSN 1615-9853. - eISSN 1615-9861
Zusammenfassung
Non-model organisms represent the majority of life forms in our planet. However, the lack of genetic information hinders us to understand the unique biological phenomena in non-model organisms at the molecular level. In this study, we applied a tandem transcriptome and proteome profiling on a non-model marine fouling organism, Bugula neritina. Using a 454 pyrosequencing platform with the updated titanium reagents, we generated a total of 48M bp transcriptome data consisting of 131 450 high-quality reads. Of these, 122 650 reads (93%) were assembled to produce 6392 contigs with an average length of 538 bases and the remaining 8800 reads were singletons. Of the total 15 192 unigenes, 13 863 ORFs were predicated, of which 6917 were functionally annotated based on gene ontology and eukaryotic orthologous groups. Subsequent proteome analysis identified and quantified 882 proteins from B. neritina. These results would provide fundamental and important information for the subsequent studies of molecular mechanism in larval biology, development, antifouling research. Furthermore, we demonstrated, for the first time, the combined use of two high-throughput technologies as a powerful approach for accelerating the studies of non-model but otherwise important species.
Zusammenfassung in einer weiteren Sprache
Fachgebiet (DDC)
570 Biowissenschaften, Biologie
Schlagwörter
Animal proteomics,Bugula neritina,Larval metamorphosis,Pyrosequencing,Transcriptome
Konferenz
Rezension
undefined / . - undefined, undefined. - (undefined; undefined)
Zitieren
ISO 690WANG, Hao, Huoming ZHANG, Yue Him WONG, Christian R. VOOLSTRA, Timothy RAVASI, Vladimir B. BAJIC, Pei-Yuan QIAN, 2010. Rapid transcriptome and proteome profiling of a non-model marine invertebrate, Bugula neritina. In: PROTEOMICS. Wiley. 10(16), pp. 2972-2981. ISSN 1615-9853. eISSN 1615-9861. Available under: doi: 10.1002/pmic.201000056
BibTex
@article{Wang2010Rapid-66479,
  year={2010},
  doi={10.1002/pmic.201000056},
  title={Rapid transcriptome and proteome profiling of a non-model marine invertebrate, Bugula neritina},
  number={16},
  volume={10},
  issn={1615-9853},
  journal={PROTEOMICS},
  pages={2972--2981},
  author={Wang, Hao and Zhang, Huoming and Wong, Yue Him and Voolstra, Christian R. and Ravasi, Timothy and B. Bajic, Vladimir and Qian, Pei-Yuan}
}
RDF
<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/66479">
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:language>eng</dc:language>
    <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/66479"/>
    <dc:creator>Zhang, Huoming</dc:creator>
    <dc:creator>B. Bajic, Vladimir</dc:creator>
    <dc:creator>Qian, Pei-Yuan</dc:creator>
    <dc:creator>Wang, Hao</dc:creator>
    <dcterms:title>Rapid transcriptome and proteome profiling of a non-model marine invertebrate, Bugula neritina</dcterms:title>
    <dcterms:abstract>Non-model organisms represent the majority of life forms in our planet. However, the lack of genetic information hinders us to understand the unique biological phenomena in non-model organisms at the molecular level. In this study, we applied a tandem transcriptome and proteome profiling on a non-model marine fouling organism, Bugula neritina. Using a 454 pyrosequencing platform with the updated titanium reagents, we generated a total of 48M bp transcriptome data consisting of 131 450 high-quality reads. Of these, 122 650 reads (93%) were assembled to produce 6392 contigs with an average length of 538 bases and the remaining 8800 reads were singletons. Of the total 15 192 unigenes, 13 863 ORFs were predicated, of which 6917 were functionally annotated based on gene ontology and eukaryotic orthologous groups. Subsequent proteome analysis identified and quantified 882 proteins from B. neritina. These results would provide fundamental and important information for the subsequent studies of molecular mechanism in larval biology, development, antifouling research. Furthermore, we demonstrated, for the first time, the combined use of two high-throughput technologies as a powerful approach for accelerating the studies of non-model but otherwise important species.</dcterms:abstract>
    <dc:creator>Wong, Yue Him</dc:creator>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:contributor>B. Bajic, Vladimir</dc:contributor>
    <dc:contributor>Qian, Pei-Yuan</dc:contributor>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2023-03-28T13:22:07Z</dcterms:available>
    <dc:creator>Voolstra, Christian R.</dc:creator>
    <dc:contributor>Ravasi, Timothy</dc:contributor>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2023-03-28T13:22:07Z</dc:date>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dcterms:issued>2010</dcterms:issued>
    <dc:contributor>Wong, Yue Him</dc:contributor>
    <dc:contributor>Voolstra, Christian R.</dc:contributor>
    <dc:contributor>Wang, Hao</dc:contributor>
    <dc:creator>Ravasi, Timothy</dc:creator>
    <dc:contributor>Zhang, Huoming</dc:contributor>
  </rdf:Description>
</rdf:RDF>
Interner Vermerk
xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter
Kontakt
URL der Originalveröffentl.
Prüfdatum der URL
Prüfungsdatum der Dissertation
Finanzierungsart
Kommentar zur Publikation
Allianzlizenz
Corresponding Authors der Uni Konstanz vorhanden
Internationale Co-Autor:innen
Universitätsbibliographie
Begutachtet
Ja