Negative Changes of the Membrane Capacitance due to Electrogenic Na Transport by the Na, K-ATPase

Sokolov, Valerij S.; Lenz, Alexander A.; Apell, Hans-Jürgen

Negative Changes of the Membrane Capacitance due to Electrogenic Na Transport by the Na, K-ATPase

Dateien

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Datum

2003

Autor:innen

Sokolov, Valerij S.

Lenz, Alexander A.

Apell, Hans-Jürgen

Open Access-Veröffentlichung

Open Access Green

Sammlungen

Biologie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

Annals of the New York Academy of Science. 2003, 986(1), pp. 229-231. ISSN 0077-8923. eISSN 1749-6632. Available under: doi: 10.1111/j.1749-6632.2003.tb07166.x

Zusammenfassung

Electrogenic Na+ transport was investigated in membrane fragments containing Na,K-ATPase adsorbed to bilayer lipid membranes (BLM) triggered by fast ATP release from caged ATP. The influence of voltage on the transport after ATP release was determined as small increaments of capacitance and conductance by applying an alternating voltage to the membrane. An electrogenic Na+ transport through a cytoplasmic access channel of the Na,K-ATPase was detected that disappeared after enzyme phosphorylation, and thus produced a negative capacitance and conductance increaments at Na+ concentrations below 5 mM3. This effect was studied now in more detail by measuring the frequency dependence of the capacitance and conductance increaments at different Na+ concentrations.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Schlagwörter

Na,K-ATPase, electrogenicity, capacitance, Na+ movements, ion binding

Zitieren

ISO 690

SOKOLOV, Valerij S., Alexander A. LENZ, Hans-Jürgen APELL, 2003. Negative Changes of the Membrane Capacitance due to Electrogenic Na Transport by the Na, K-ATPase. In: Annals of the New York Academy of Science. 2003, 986(1), pp. 229-231. ISSN 0077-8923. eISSN 1749-6632. Available under: doi: 10.1111/j.1749-6632.2003.tb07166.x

BibTex

@article{Sokolov2003Negat-8187,
  year={2003},
  doi={10.1111/j.1749-6632.2003.tb07166.x},
  title={Negative Changes of the Membrane Capacitance due to Electrogenic Na Transport by the Na, K-ATPase},
  number={1},
  volume={986},
  issn={0077-8923},
  journal={Annals of the New York Academy of Science},
  pages={229--231},
  author={Sokolov, Valerij S. and Lenz, Alexander A. and Apell, Hans-Jürgen}
}

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