Controlling and measuring dynamic odorant stimuli in the laboratory

Gorur-Shandilya, Srinivas; Martelli, Carlotta; Demir, Mahmut; Emonet, Thierry

Controlling and measuring dynamic odorant stimuli in the laboratory

Dateien

Zu diesem Dokument gibt es keine Dateien.

Datum

2019

Autor:innen

Gorur-Shandilya, Srinivas

Martelli, Carlotta

Demir, Mahmut

Emonet, Thierry

DOI (zitierfähiger Link)

https://doi.org/10.1242/jeb.207787

Sammlungen

Biologie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

The Journal of Experimental Biology. 2019, 222(23), jeb207787. ISSN 0022-0949. eISSN 1477-9145. Available under: doi: 10.1242/jeb.207787

Zusammenfassung

Animals experience complex odorant stimuli that vary widely in composition, intensity and temporal properties. However, stimuli used to study olfaction in the laboratory are much simpler. This mismatch arises from the challenges in measuring and controlling them precisely and accurately. Even simple pulses can have diverse kinetics that depend on their molecular identity. Here, we introduce a model that describes how stimulus kinetics depend on the molecular identity of the odorant and the geometry of the delivery system. We describe methods to deliver dynamic odorant stimuli of several types, including broadly distributed stimuli that reproduce some of the statistics of naturalistic plumes, in a reproducible and precise manner. Finally, we introduce a method to calibrate a photo-ionization detector to any odorant it can detect, using no additional components. Our approaches are affordable and flexible and can be used to advance our understanding of how olfactory neurons encode real-world odor signals.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Schlagwörter

Mass flow controller, Photo-ionization detector, Automation, Calibration, Odor control, Olfaction

Zitieren

ISO 690

GORUR-SHANDILYA, Srinivas, Carlotta MARTELLI, Mahmut DEMIR, Thierry EMONET, 2019. Controlling and measuring dynamic odorant stimuli in the laboratory. In: The Journal of Experimental Biology. 2019, 222(23), jeb207787. ISSN 0022-0949. eISSN 1477-9145. Available under: doi: 10.1242/jeb.207787

BibTex

@article{GorurShandilya2019-11-29Contr-48288,
  year={2019},
  doi={10.1242/jeb.207787},
  title={Controlling and measuring dynamic odorant stimuli in the laboratory},
  number={23},
  volume={222},
  issn={0022-0949},
  journal={The Journal of Experimental Biology},
  author={Gorur-Shandilya, Srinivas and Martelli, Carlotta and Demir, Mahmut and Emonet, Thierry},
  note={Article Number: jeb207787}
}

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Universitätsbibliographie

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