The Effect of Water Hardness on Mortality of Zebrafish (Danio rerio) During Exposure to Oxytetracycline

Hundt, Matthias; Schreiber, Benjamin; Eckmann, Reiner; Lunestad, Bjørn Tore; Wünneman, Hannah; Schulz, Ralf

The Effect of Water Hardness on Mortality of Zebrafish (Danio rerio) During Exposure to Oxytetracycline

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Date

2016

Authors

Hundt, Matthias

Schreiber, Benjamin

Eckmann, Reiner

Lunestad, Bjørn Tore

Wünneman, Hannah

Schulz, Ralf

DOI (citable link)

10.1007/s00128-015-1699-x

Collections

Biologie

Publication type

Journal article

Publication status

Published

Published in

Bulletin of Environmental Contamination and Toxicology ; 96 (2016), 2. - pp. 144-149. - ISSN 0007-4861. - eISSN 1432-0800

Abstract

Marking of fish otoliths with oxytetracycline and tetracycline is a widely used method to evaluate the effectiveness of stocking operations. Available protocols for the labeling of fish specify a number of factors influencing mark quality and potential risk for fish during marking. This study investigates the influence of water hardness on mortality of freshwater fish during marking with OTC. In order to pursue this question complexation of OTC with Mg²⁺ and Ca²⁺cations was measured spectrophotometrically. Furthermore, zebrafish (Danio rerio) were immersed in OTC solutions (1200 mg/L; 48 h immersion) combined with varying levels of water hardness (5.5, 15.5, 25.5, 32.5°dH). The amount of OTC-Mg-Ca-complexes was positively correlated to water hardness. Moreover, it could be demonstrated that mortality of zebrafish during marking varied as a factor of water hardness. Highest mortalities occurred at the lowest (5.5°dH) and the highest (32.5°dH) tested levels during marking with OTC.

Subject (DDC)

570 Biosciences, Biology

Keywords

Oxytetracycline, Tetracycline, Water hardness, Danio rerio, Restocking, Immersion marking

Cite This

ISO 690

HUNDT, Matthias, Benjamin SCHREIBER, Reiner ECKMANN, Bjørn Tore LUNESTAD, Hannah WÜNNEMAN, Ralf SCHULZ, 2016. The Effect of Water Hardness on Mortality of Zebrafish (Danio rerio) During Exposure to Oxytetracycline. In: Bulletin of Environmental Contamination and Toxicology. 96(2), pp. 144-149. ISSN 0007-4861. eISSN 1432-0800. Available under: doi: 10.1007/s00128-015-1699-x

BibTex

@article{Hundt2016Effec-33626,
  year={2016},
  doi={10.1007/s00128-015-1699-x},
  title={The Effect of Water Hardness on Mortality of Zebrafish (Danio rerio) During Exposure to Oxytetracycline},
  number={2},
  volume={96},
  issn={0007-4861},
  journal={Bulletin of Environmental Contamination and Toxicology},
  pages={144--149},
  author={Hundt, Matthias and Schreiber, Benjamin and Eckmann, Reiner and Lunestad, Bjørn Tore and Wünneman, Hannah and Schulz, Ralf}
}

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Bibliography of Konstanz

Yes