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Peridinin–chlorophyll–protein reconstituted with chlorophyll mixtures : Preparation, bulk and single molecule spectroscopy

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2006

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Brotosudarmo, Tatas H. P.
Hofmann, Eckhard
Hiller, Roger G.
Wörmke, Stephan
Mackowski, Sebastian
Bräuchle, Christoph
Scheer, Hugo

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https://doi.org/10.1016/j.febslet.2006.08.049
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FEBS Letters. 2006, 580(22), pp. 5257-5262. ISSN 0014-5793. eISSN 1873-3468. Available under: doi: 10.1016/j.febslet.2006.08.049

Zusammenfassung

Reconstitution of the 16 kDa N-terminal domain of the peridinin-chlorophyll-protein, N-PCP, with mixtures of chlorophyll a (Chl a) and Chl b, resulted in 32 kDa complexes containing two pigment clusters, each bound to one N-PCP. Besides homo-chlorophyllous complexes, hetero-chlorophyllous ones were obtained that contain Chl a in one pigment cluster, and Chl b in the other. Binding of Chl b is stronger than that of the native pigment, Chl a. Energy transfer from Chl b to Chl a is efficient, but there are only weak interactions between the two pigments. Individual homo- and hetero-chlorophyllous complexes were investigated by single molecule spectroscopy using excitation into the peridinin absorption band and scanning of the Chl fluorescence, the latter show frequently well resolved emissions of the two pigments.

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Fachgebiet (DDC)
540 Chemie

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Photosynthesis; Light-harvesting; Chlorophyll; Carotenoid; Energy transfer; Single molecule spectroscopy

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ISO 690BROTOSUDARMO, Tatas H. P., Eckhard HOFMANN, Roger G. HILLER, Stephan WÖRMKE, Sebastian MACKOWSKI, Andreas ZUMBUSCH, Christoph BRÄUCHLE, Hugo SCHEER, 2006. Peridinin–chlorophyll–protein reconstituted with chlorophyll mixtures : Preparation, bulk and single molecule spectroscopy. In: FEBS Letters. 2006, 580(22), pp. 5257-5262. ISSN 0014-5793. eISSN 1873-3468. Available under: doi: 10.1016/j.febslet.2006.08.049
BibTex
@article{Brotosudarmo2006-10-02Perid-42307,
  year={2006},
  doi={10.1016/j.febslet.2006.08.049},
  title={Peridinin–chlorophyll–protein reconstituted with chlorophyll mixtures : Preparation, bulk and single molecule spectroscopy},
  number={22},
  volume={580},
  issn={0014-5793},
  journal={FEBS Letters},
  pages={5257--5262},
  author={Brotosudarmo, Tatas H. P. and Hofmann, Eckhard and Hiller, Roger G. and Wörmke, Stephan and Mackowski, Sebastian and Zumbusch, Andreas and Bräuchle, Christoph and Scheer, Hugo}
}
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