Publikation:

High sensitivity stimulated Raman scattering microscopy with electronic resonance enhancement

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2024

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URI (zitierfähiger Link)
http://nbn-resolving.de/urn:nbn:de:bsz:352-2-xqqrb9z73mzu6
DOI (zitierfähiger Link)
https://doi.org/10.1002/cphc.202400309
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oops

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European Union (EU): 812922

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Open Access-Veröffentlichung
Open Access Hybrid

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Chemie: Publikationen
Core Facility der Universität Konstanz

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Published

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ChemPhysChem. Wiley. 2024, 25(19), e202400309. ISSN 1439-4235. eISSN 1439-7641. Verfügbar unter: doi: 10.1002/cphc.202400309

Zusammenfassung

Raman microscopy is an important tool for labelfree microscopy. However, spontaneous Raman microscopy suffers from slow image acquisition rates and susceptibility to fluorescence background. Coherent Raman microsocopy techniques such as coherent anti‐Stokes Raman scattering (CARS) and stimulated Raman scattering (SRS) microscopy, by contrast, offer fast imaging capability and robustness against sample fluorescence. Yet, their rather low sensitivity impedes their broader application. This review discusses sensitivity enhancement of SRS microscopy to µM detection levels by using electronically pre‐resonant excitation. We present the foundations of this approach, discuss its technological implementation, and show first successful applications. A special emphasis is given to outlining new experimental developments allowing novel types of investigations.

Zusammenfassung in einer weiteren Sprache

Fachgebiet (DDC)
540 Chemie

Schlagwörter

Raman scattering, Raman microscopy, non-linear spectroscopy, bioimaging, non-linear microscopy

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ISO 690PRUCCOLI, Andrea, Andreas ZUMBUSCH, 2024. High sensitivity stimulated Raman scattering microscopy with electronic resonance enhancement. In: ChemPhysChem. Wiley. 2024, 25(19), e202400309. ISSN 1439-4235. eISSN 1439-7641. Verfügbar unter: doi: 10.1002/cphc.202400309
BibTex
@article{Pruccoli2024-10sensi-70656,
  title={High sensitivity stimulated Raman scattering microscopy with electronic resonance enhancement},
  year={2024},
  doi={10.1002/cphc.202400309},
  number={19},
  volume={25},
  issn={1439-4235},
  journal={ChemPhysChem},
  author={Pruccoli, Andrea and Zumbusch, Andreas},
  note={Article Number: e202400309}
}
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