Publikation:

Unifying bacteria from decaying wood with various ubiquitous Gibbsiella species as G. acetica sp. nov. based on nucleotide sequence similarities and their acetic acid secretion

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2015

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Geider, Klaus
Gernold, Marina
Jock, Susanne
Wensing, Annette
Völksch, Beate
Gross, Jürgen

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http://nbn-resolving.de/urn:nbn:de:bsz:352-0-292043
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https://doi.org/10.1016/j.micres.2015.05.003
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Biologie: Publikationen
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Microbiological Research. 2015, 181, pp. 93-104. ISSN 0944-5013. eISSN 1618-0623. Available under: doi: 10.1016/j.micres.2015.05.003

Zusammenfassung

Bacteria were isolated from necrotic apple and pear tree tissue and from dead wood in Germany and Austria as well as from pear tree exudate in China. They were selected for growth at 37 °C, screened for levan production and then characterized as Gram-negative, facultatively anaerobic rods. Nucleotide sequences from 16S rRNA genes, the housekeeping genes dnaJ, gyrB, recA and rpoB. Alignments, BLAST searches and phenotypic data confirmed by MALDI-TOF analysis showed that these bacteria belong to the genus Gibbsiella and resembled strains isolated from diseased oaks in Britain and Spain. Gibbsiella-specific PCR primers were designed from the proline isomerase and the levansucrase genes. Acid secretion was investigated by screening for halo formation on calcium carbonate agar and the compound identified by NMR as acetic acid. Its production by Gibbsiella spp. strains was also determined in culture supernatants by GC/MS analysis after derivatization with pentafluorobenzyl bromide. Some strains were differentiated by the PFGE patterns of SpeI digests and by sequence analyses of the lsc and the ppiD genes, and the Chinese Gibbsiella strain was most divergent. The newly investigated bacteria as well as G. querinecans, G. dentisursi and G. papilionis, isolated in Britain, Spain, Korea and Japan, are taxonomicall relatedy Enterobacteriaceae, tolerate and secrete acetic acid. We therefore propose to unify them in the species Gibbsiella acetica sp. nov.

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570 Biowissenschaften, Biologie

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ISO 690GEIDER, Klaus, Marina GERNOLD, Susanne JOCK, Annette WENSING, Beate VÖLKSCH, Jürgen GROSS, Dieter SPITELLER, 2015. Unifying bacteria from decaying wood with various ubiquitous Gibbsiella species as G. acetica sp. nov. based on nucleotide sequence similarities and their acetic acid secretion. In: Microbiological Research. 2015, 181, pp. 93-104. ISSN 0944-5013. eISSN 1618-0623. Available under: doi: 10.1016/j.micres.2015.05.003
BibTex
@article{Geider2015-12Unify-31077,
  year={2015},
  doi={10.1016/j.micres.2015.05.003},
  title={Unifying bacteria from decaying wood with various ubiquitous Gibbsiella species as G. acetica sp. nov. based on nucleotide sequence similarities and their acetic acid secretion},
  volume={181},
  issn={0944-5013},
  journal={Microbiological Research},
  pages={93--104},
  author={Geider, Klaus and Gernold, Marina and Jock, Susanne and Wensing, Annette and Völksch, Beate and Gross, Jürgen and Spiteller, Dieter}
}
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    <dcterms:abstract xml:lang="eng">Bacteria were isolated from necrotic apple and pear tree tissue and from dead wood in Germany and Austria as well as from pear tree exudate in China. They were selected for growth at 37 °C, screened for levan production and then characterized as Gram-negative, facultatively anaerobic rods. Nucleotide sequences from 16S rRNA genes, the housekeeping genes dnaJ, gyrB, recA and rpoB. Alignments, BLAST searches and phenotypic data confirmed by MALDI-TOF analysis showed that these bacteria belong to the genus Gibbsiella and resembled strains isolated from diseased oaks in Britain and Spain. Gibbsiella-specific PCR primers were designed from the proline isomerase and the levansucrase genes. Acid secretion was investigated by screening for halo formation on calcium carbonate agar and the compound identified by NMR as acetic acid. Its production by Gibbsiella spp. strains was also determined in culture supernatants by GC/MS analysis after derivatization with pentafluorobenzyl bromide. Some strains were differentiated by the PFGE patterns of SpeI digests and by sequence analyses of the lsc and the ppiD genes, and the Chinese Gibbsiella strain was most divergent. The newly investigated bacteria as well as G. querinecans, G. dentisursi and G. papilionis, isolated in Britain, Spain, Korea and Japan, are taxonomicall relatedy Enterobacteriaceae, tolerate and secrete acetic acid. We therefore propose to unify them in the species Gibbsiella acetica sp. nov.</dcterms:abstract>
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