Publikation:

Intracellularly inducible, ubiquitin hydrolase-insensitive tandem ubiquitins inhibit the 26S proteasome activity and cell division

Lade...
Vorschaubild

Dateien

Zu diesem Dokument gibt es keine Dateien.

Datum

2004

Autor:innen

Saeki, Yasushi
Oguchi, Tomoko
Shimada, Masumi
Sone, Takayuki
Kawahara, Hiroyuki
Yokosawa, Hideyoshi
Toh-e, Akio

Herausgeber:innen

Kontakt

ISSN der Zeitschrift

Electronic ISSN

ISBN

Bibliografische Daten

Verlag

Schriftenreihe

Auflagebezeichnung

URI (zitierfähiger Link)
DOI (zitierfähiger Link)
ArXiv-ID

Internationale Patentnummer

Angaben zur Forschungsförderung

Projekt

Open Access-Veröffentlichung
Core Facility der Universität Konstanz

Gesperrt bis

Titel in einer weiteren Sprache

Publikationstyp
Zeitschriftenartikel
Publikationsstatus
Published

Erschienen in

Genes & Genetic Systems. 2004, 79(2), pp. 77-86. ISSN 1341-7568. eISSN 1880-5779. Available under: doi: 10.1266/ggs.79.77

Zusammenfassung

We constructed polyubiquitin derivatives that contain a tandem repeat of ubiquitins and were insensitive to ubiquitin hydrolases. They were designated tandem ubiquitin (tUb) with the number of repeats, such as tUb2. When tUbs were expressed under the control of the GAL1 promoter in the wild-type yeast strain, growth was strongly inhibited. Under these conditions, the degradation of N-end rule substrates, a UFD substrate and Gcn4 was inhibited, indicating that the tUb inhibits 26S proteasome activity. Consistent with this, tUb binds to the 26S proteasome. We showed that tUb inhibited the in vitro degradation of polyubiquitinylated Sic1 by the 26S proteasome. When tUB6 messenger RNA was injected into Xenopus embryos, cell division was inhibited, suggesting that tUb can be used as a versatile inhibitor of the 26S proteasome.

Zusammenfassung in einer weiteren Sprache

Fachgebiet (DDC)
570 Biowissenschaften, Biologie

Schlagwörter

hydrolase resistant-tandem ubiquitin, inhibitor, proteasome, proteolysis, yeast

Konferenz

Rezension
undefined / . - undefined, undefined

Forschungsvorhaben

Organisationseinheiten

Zeitschriftenheft

Zugehörige Datensätze in KOPS

Zitieren

ISO 690SAEKI, Yasushi, Erika ISONO, Tomoko OGUCHI, Masumi SHIMADA, Takayuki SONE, Hiroyuki KAWAHARA, Hideyoshi YOKOSAWA, Akio TOH-E, 2004. Intracellularly inducible, ubiquitin hydrolase-insensitive tandem ubiquitins inhibit the 26S proteasome activity and cell division. In: Genes & Genetic Systems. 2004, 79(2), pp. 77-86. ISSN 1341-7568. eISSN 1880-5779. Available under: doi: 10.1266/ggs.79.77
BibTex
@article{Saeki2004Intra-42153,
  year={2004},
  doi={10.1266/ggs.79.77},
  title={Intracellularly inducible, ubiquitin hydrolase-insensitive tandem ubiquitins inhibit the 26S proteasome activity and cell division},
  number={2},
  volume={79},
  issn={1341-7568},
  journal={Genes & Genetic Systems},
  pages={77--86},
  author={Saeki, Yasushi and Isono, Erika and Oguchi, Tomoko and Shimada, Masumi and Sone, Takayuki and Kawahara, Hiroyuki and Yokosawa, Hideyoshi and Toh-e, Akio}
}
RDF
<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/42153">
    <dc:contributor>Kawahara, Hiroyuki</dc:contributor>
    <dc:creator>Saeki, Yasushi</dc:creator>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:contributor>Oguchi, Tomoko</dc:contributor>
    <dc:creator>Shimada, Masumi</dc:creator>
    <dc:creator>Sone, Takayuki</dc:creator>
    <dc:creator>Kawahara, Hiroyuki</dc:creator>
    <dc:contributor>Sone, Takayuki</dc:contributor>
    <dc:creator>Yokosawa, Hideyoshi</dc:creator>
    <dcterms:issued>2004</dcterms:issued>
    <dcterms:abstract xml:lang="eng">We constructed polyubiquitin derivatives that contain a tandem repeat of ubiquitins and were insensitive to ubiquitin hydrolases. They were designated tandem ubiquitin (tUb) with the number of repeats, such as tUb2. When tUbs were expressed under the control of the GAL1 promoter in the wild-type yeast strain, growth was strongly inhibited. Under these conditions, the degradation of N-end rule substrates, a UFD substrate and Gcn4 was inhibited, indicating that the tUb inhibits 26S proteasome activity. Consistent with this, tUb binds to the 26S proteasome. We showed that tUb inhibited the in vitro degradation of polyubiquitinylated Sic1 by the 26S proteasome. When tUB6 messenger RNA was injected into Xenopus embryos, cell division was inhibited, suggesting that tUb can be used as a versatile inhibitor of the 26S proteasome.</dcterms:abstract>
    <dc:contributor>Isono, Erika</dc:contributor>
    <dc:contributor>Shimada, Masumi</dc:contributor>
    <dc:contributor>Toh-e, Akio</dc:contributor>
    <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/42153"/>
    <dc:contributor>Yokosawa, Hideyoshi</dc:contributor>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2018-04-24T12:53:14Z</dcterms:available>
    <dc:contributor>Saeki, Yasushi</dc:contributor>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:creator>Isono, Erika</dc:creator>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dc:creator>Toh-e, Akio</dc:creator>
    <dcterms:title>Intracellularly inducible, ubiquitin hydrolase-insensitive tandem ubiquitins inhibit the 26S proteasome activity and cell division</dcterms:title>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2018-04-24T12:53:14Z</dc:date>
    <dc:language>eng</dc:language>
    <dc:creator>Oguchi, Tomoko</dc:creator>
  </rdf:Description>
</rdf:RDF>

Interner Vermerk

xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter

Kontakt
URL der Originalveröffentl.

Prüfdatum der URL

Prüfungsdatum der Dissertation

Finanzierungsart

Kommentar zur Publikation

Allianzlizenz
Corresponding Authors der Uni Konstanz vorhanden
Internationale Co-Autor:innen
Universitätsbibliographie
Nein
Begutachtet
Diese Publikation teilen