Publikation:

A multigene family encoding R-SNAREs in the ciliate Paramecium tetraurelia

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2006

Autor:innen

Schilde, Christina
Wassmer, Thomas
Mansfeld, Joerg

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URI (zitierfähiger Link)
http://nbn-resolving.de/urn:nbn:de:bsz:352-opus-43179
DOI (zitierfähiger Link)
https://doi.org/10.1111/j.1600-0854.2006.00397.x
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Attribution-NonCommercial-NoDerivs 2.0 Generic

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Open Access-Veröffentlichung
Open Access Green

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Biologie: Publikationen
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Zeitschriftenartikel
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Published

Erschienen in

Traffic. 2006, 7(4), pp. 440-455. ISSN 1398-9219. eISSN 1398-9219. Available under: doi: 10.1111/j.1600-0854.2006.00397.x

Zusammenfassung

SNARE proteins (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) mediate membrane interactions and are conventionally divided into Q-SNAREs and R-SNAREs according to the possession of a glutamine or arginine residue at the core of their SNARE domain. Here, we describe a set of R-SNAREs from the ciliate Paramecium tetraurelia consisting of seven families encoded by 12 genes that are expressed simultaneously. The complexity of the endomembrane system in Paramecium can explain this high number of genes. All P. tetraurelia synaptobrevins (PtSybs) possess a SNARE domain and show homology to the Longin family of R-SNAREs such as Ykt6, Sec22 and tetanus toxin-insensitive VAMP (TI-VAMP). We localized four exemplary PtSyb subfamilies with GFP constructs and antibodies on the light and electron microscopic level. PtSyb1-1, PtSyb1-2 and PtSyb3-1 were found in the endoplasmic reticulum, whereas PtSyb2 is localized exclusively in the contractile vacuole complex. PtSyb6 was found cytosolic but also resides in regularly arranged structures at the cell cortex (parasomal sacs), the cytoproct and oral apparatus, probably representing endocytotic compartments. With gene silencing, we showed that the R-SNARE of the contractile vacuole complex, PtSyb2, functions to maintain structural integrity as well as functionality of the osmoregulatory system but also affects cell division.

Zusammenfassung in einer weiteren Sprache

Fachgebiet (DDC)
570 Biowissenschaften, Biologie

Schlagwörter

ciliates, membrane trafficking, Paramecium, regulated secretion, SNAREs, R-SNAREs, v-SNAREs

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ISO 690SCHILDE, Christina, Thomas WASSMER, Joerg MANSFELD, Helmut PLATTNER, Roland KISSMEHL, 2006. A multigene family encoding R-SNAREs in the ciliate Paramecium tetraurelia. In: Traffic. 2006, 7(4), pp. 440-455. ISSN 1398-9219. eISSN 1398-9219. Available under: doi: 10.1111/j.1600-0854.2006.00397.x
BibTex
@article{Schilde2006multi-7401,
  year={2006},
  doi={10.1111/j.1600-0854.2006.00397.x},
  title={A multigene family encoding R-SNAREs in the ciliate Paramecium tetraurelia},
  number={4},
  volume={7},
  issn={1398-9219},
  journal={Traffic},
  pages={440--455},
  author={Schilde, Christina and Wassmer, Thomas and Mansfeld, Joerg and Plattner, Helmut and Kissmehl, Roland}
}
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