Publikation: Kinetic Investigations of the Mechanism of the Rate-Determing Step of the Na+, K+ -ATPase Pump Cycle
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2003
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Annals of the New York Academy of Science. 2003, 986(1), pp. 159-162. ISSN 0077-8923. eISSN 1749-6632. Available under: doi: 10.1111/j.1749-6632.2003.tb07154.x
Zusammenfassung
The kinetics of the E2->E1 conformational change of unphosphorylated Na+ ,K+ -ATPase from rabbit kidney were investigated via the stopped-flow technique using the fluorescent label RH421 (pH 7.4, 24°C). The enzyme was preequilibrated in a solution containing 25 mM histidine and 0.1 mM EDTA to initially stabilize the E2 conformation. On mixing enzyme with NaCl alone, tris-ATP alone, or NaCl and tris-ATP simultaneously, a fluorescence decrease was observed.
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Fachgebiet (DDC)
570 Biowissenschaften, Biologie
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stopped-flow, fluorescence, voltage-sensitive dye, rate constant, regulation
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CLARKE, Ronald J., Paul HUMPHREY, Christian LÜPFERT, Hans-Jürgen APELL, Flemming CORNELIUS, 2003. Kinetic Investigations of the Mechanism of the Rate-Determing Step of the Na+, K+ -ATPase Pump Cycle. In: Annals of the New York Academy of Science. 2003, 986(1), pp. 159-162. ISSN 0077-8923. eISSN 1749-6632. Available under: doi: 10.1111/j.1749-6632.2003.tb07154.xBibTex
@article{Clarke2003Kinet-7310,
year={2003},
doi={10.1111/j.1749-6632.2003.tb07154.x},
title={Kinetic Investigations of the Mechanism of the Rate-Determing Step of the Na+, K+ -ATPase Pump Cycle},
number={1},
volume={986},
issn={0077-8923},
journal={Annals of the New York Academy of Science},
pages={159--162},
author={Clarke, Ronald J. and Humphrey, Paul and Lüpfert, Christian and Apell, Hans-Jürgen and Cornelius, Flemming}
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