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The sonic hedgehog pathway mediates carbamylated erythropoietin-enhanced proliferation and differentiation of adult neural progenitor cells

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2007

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Wang, Lei
Zhang, Zheng Gang
Gregg, Sara R.
Zhang, Rui Lan
Jiao, Zhongxian
LeTourneau, Yvonne
Liu, Xianshuang
Feng, Yifan
Gerwien, Jens
Torup, Lars
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http://nbn-resolving.de/urn:nbn:de:bsz:352-opus-119687
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https://doi.org/10.1074/jbc.M706880200
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Published

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Journal of Biological Chemistry. 2007, 282(44), pp. 32462-32470. ISSN 0021-9258. Available under: doi: 10.1074/jbc.M706880200

Zusammenfassung

Carbamylated erythropoietin (CEPO), a well characterized erythropoietin (EPO) derivative, does not bind to the classical EPO receptor and does not stimulate erythropoiesis. Using neural progenitor cells derived from the subventricular zone of the adult mouse, we investigated the effect of CEPO on neurogenesis and the associated signaling pathways in vitro. We found that CEPO significantly increased neural progenitor cell proliferation and promoted neural progenitor cell differentiation into neurons, which was associated with up-regulation of Sonic hedgehog (Shh), its receptor ptc, and mammalian achaete-scute homolog 1 (Mash1), a pro-neuron basic helix-loop-helix protein transcription factor. Blockage of the Shh signaling pathway with a pharmacological inhibitor, cyclopamine, abolished the CEPO-induced neurogenesis. Attenuation of endogenous Mash1 expression by short-interfering RNA blocked CEPO-promoted neuronal differentiation. In addition, recombinant mouse Shh up-regulated Mash1 expression in neural progenitor cells. These results demonstrate that the Shh signaling pathway mediates CEPO-enhanced neurogenesis and Mash1 is a downstream target of the Shh signaling pathway that regulates CEPO-enhanced neuronal differentiation.

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570 Biowissenschaften, Biologie

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ISO 690WANG, Lei, Zheng Gang ZHANG, Sara R. GREGG, Rui Lan ZHANG, Zhongxian JIAO, Yvonne LETOURNEAU, Xianshuang LIU, Yifan FENG, Jens GERWIEN, Lars TORUP, Marcel LEIST, Constance Tom NOGUCHI, Zhi-Yong CHEN, Michael CHOPP, 2007. The sonic hedgehog pathway mediates carbamylated erythropoietin-enhanced proliferation and differentiation of adult neural progenitor cells. In: Journal of Biological Chemistry. 2007, 282(44), pp. 32462-32470. ISSN 0021-9258. Available under: doi: 10.1074/jbc.M706880200
BibTex
@article{Wang2007-11-02sonic-1172,
  year={2007},
  doi={10.1074/jbc.M706880200},
  title={The sonic hedgehog pathway mediates carbamylated erythropoietin-enhanced proliferation and differentiation of adult neural progenitor cells},
  number={44},
  volume={282},
  issn={0021-9258},
  journal={Journal of Biological Chemistry},
  pages={32462--32470},
  author={Wang, Lei and Zhang, Zheng Gang and Gregg, Sara R. and Zhang, Rui Lan and Jiao, Zhongxian and LeTourneau, Yvonne and Liu, Xianshuang and Feng, Yifan and Gerwien, Jens and Torup, Lars and Leist, Marcel and Noguchi, Constance Tom and Chen, Zhi-Yong and Chopp, Michael}
}
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