In vitro research reproducibility : Keeping up high standards

Hirsch, Cordula; Schildknecht, Stefan

In vitro research reproducibility : Keeping up high standards

Dateien

Hirsch_2-10e6snhkxwr5q6.pdfGröße: 1010.35 KBDownloads: 203

Datum

2019

Autor:innen

Hirsch, Cordula

Schildknecht, Stefan

EU-Projektnummer

681002

Projekt

EUToxRisk21

Sammlungen

Biologie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

Frontiers in Pharmacology. 2019, 10, 1484. eISSN 1663-9812. Available under: doi: 10.3389/fphar.2019.01484

Zusammenfassung

Concern regarding the reproducibility of observations in life science research has emerged in recent years, particularly in view of unfavorable experiences with preclinical in vivo research. The use of cell-based systems has increasingly replaced in vivo research and the application of in vitro models enjoys an ever-growing popularity. To avoid repeating past mistakes, high standards of reproducibility and reliability must be established and maintained in the field of in vitro biomedical research. Detailed guidance documenting the appropriate handling of cells has been authored but was received with quite disparate perception by different branches in biomedical research. In that regard, we intend to raise awareness of the reproducibility issue among scientists in all branches of contemporary life science research and their individual responsibility in this matter. We have herein compiled a selection of the most susceptible steps of everyday in vitro cell culture routines that have the potential to influence cell quality and recommend practices to minimize the likelihood of poor cell quality impairing reproducibility with modest investment of time and resources.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Schlagwörter

Cell culture models, reproducibility, Toxicology, Good cell culture practice, New Approach Methods (NAM) 2

Zitieren

ISO 690

HIRSCH, Cordula, Stefan SCHILDKNECHT, 2019. In vitro research reproducibility : Keeping up high standards. In: Frontiers in Pharmacology. 2019, 10, 1484. eISSN 1663-9812. Available under: doi: 10.3389/fphar.2019.01484

BibTex

@article{Hirsch2019vitro-47652,
  year={2019},
  doi={10.3389/fphar.2019.01484},
  title={In vitro research reproducibility : Keeping up high standards},
  volume={10},
  journal={Frontiers in Pharmacology},
  author={Hirsch, Cordula and Schildknecht, Stefan},
  note={Article Number: 1484}
}

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Universitätsbibliographie

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Begutachtet

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