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α-carboxyethyl-6-hydroxychroman as urinary metabolite of vitamin E

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1997

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Schultz, Manfred
Elsner, Angelika
Brigelius-Flohé, Regina

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https://doi.org/10.1016/s0076-6879(97)82116-7
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Methods in Enzymology. Elsevier. 1997, 282, pp. 297-310. ISSN 0076-6879. eISSN 1557-7988. Available under: doi: 10.1016/s0076-6879(97)82116-7

Zusammenfassung

This chapter describes methods for the identification and estimation of urinary 2,5,7,8-tetramethyl-2-(2'-carboxyethyl)-6-hydroxychroman (α-CEHC) suitable to further investigate the conditions for α-CEHC excretion in humans and animals. α-CEHC is excreted as a sulfate or glucuronic acid conjugate. These conjugates must be hydrolyzed before determination to yield either the methyl esters or the free metabolites. Hydrolysis with a glucuronidase/sulfatase mixture leads to free α-CEHC. Due to the lack of a standard α-CEHC conjugate, it is not possible to test the recovery of α-CEHC released from conjugates. The conditions of enzymatic hydrolysis are therefore varied to determine if they are optimal. Increasing amounts of enzyme are used to hydrolyze a fixed amount of urine powder for increasing times, and the resulting α-CEHC is measured by means of gas chromatography/mass spectrometry (GC/MS). The hydrolysis of α-CEHC conjugates with methanolic HCl has been used to esterify the carboxylic acid derivatives of α-tocopherol and prevent lactonization.

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570 Biowissenschaften, Biologie

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ISO 690SCHULTZ, Manfred, Marcel LEIST, Angelika ELSNER, Regina BRIGELIUS-FLOHÉ, 1997. α-carboxyethyl-6-hydroxychroman as urinary metabolite of vitamin E. In: Methods in Enzymology. Elsevier. 1997, 282, pp. 297-310. ISSN 0076-6879. eISSN 1557-7988. Available under: doi: 10.1016/s0076-6879(97)82116-7
BibTex
@article{Schultz1997carbo-51295,
  year={1997},
  doi={10.1016/s0076-6879(97)82116-7},
  title={α-carboxyethyl-6-hydroxychroman as urinary metabolite of vitamin E},
  volume={282},
  issn={0076-6879},
  journal={Methods in Enzymology},
  pages={297--310},
  author={Schultz, Manfred and Leist, Marcel and Elsner, Angelika and Brigelius-Flohé, Regina}
}
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