Publikation: Functional dissection of Escherichia coli trigger factor : unraveling the function of individual domains
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In Escherichia coli, the ribosome-associated chaperone Trigger Factor (TF) promotes the folding of newly synthesized cytosolic proteins. TF is composed of three domains: an N-terminal domain (N), which mediates ribosome binding; a central domain (P), which has peptidyl-prolyl cis/trans isomerase activity and is involved in substrate binding in vitro; and a C-terminal domain (C) with unknown function. We investigated the contributions of individual domains (N, P, and C) or domain combinations (NP, PC, and NC) to the chaperone activity of TF in vivo and in vitro. All fragments comprising the N domain (N, NP, NC) complemented the synthetic lethality of Δtig ΔdnaK in cells lacking TF and DnaK, prevented protein aggregation in these cells, and cross-linked to nascent polypeptides in vitro. However, ΔtigΔdnaK cells expressing the N domain alone grew more slowly and showed less viability than ΔtigΔdnaK cells synthesizing either NP, NC, or full-length TF, indicating beneficial contributions of the P and C domains to TF s chaperone activity. In an in vitro system with purified components, none of the TF fragments assisted the refolding of denatured D-glyceraldehyde-3-phosphate dehydrogenase in a manner comparable to that of wild-type TF, suggesting that the observed chaperone activity of TF fragments in vivo is dependent on their localization at the ribosome. These results indicate that the N domain, in addition to its function to promote binding to the ribosome, has a chaperone activity per se and is sufficient to substitute for TF in vivo.
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KRAMER, Günter, Anna RUTKOWSKA, Renee D. WEGRZYN, Holger PATZELT, Thorben A. KURZ, Frieder MERZ, Thomas RAUCH, Sonja VORDERWÜLBECKE, Elke DEUERLING, Bernd BUKAU, 2004. Functional dissection of Escherichia coli trigger factor : unraveling the function of individual domains. In: Journal of Bacteriology. 2004, 186(12), pp. 3777-3784. ISSN 0021-9193. Available under: doi: 10.1128/JB.186.12.3777-3784.2004BibTex
@article{Kramer2004Funct-8506, year={2004}, doi={10.1128/JB.186.12.3777-3784.2004}, title={Functional dissection of Escherichia coli trigger factor : unraveling the function of individual domains}, number={12}, volume={186}, issn={0021-9193}, journal={Journal of Bacteriology}, pages={3777--3784}, author={Kramer, Günter and Rutkowska, Anna and Wegrzyn, Renee D. and Patzelt, Holger and Kurz, Thorben A. and Merz, Frieder and Rauch, Thomas and Vorderwülbecke, Sonja and Deuerling, Elke and Bukau, Bernd} }
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