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Improved protocol for recovery of bacterial DNA from water filters : Sonication and backflushing of commercial syringe filters

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2013

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Kesberg, Anna Isabella

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http://nbn-resolving.de/urn:nbn:de:bsz:352-223273
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https://doi.org/10.1016/j.mimet.2013.02.001
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Journal of Microbiological Methods. 2013, 93(1), pp. 55-57. ISSN 0167-7012. eISSN 1872-8359. Available under: doi: 10.1016/j.mimet.2013.02.001

Zusammenfassung

Extraction of bacterial DNA from water filters is frequently used in aquatic microbial ecology, e.g., for sequencing-based diversity studies. Here, we describe an improved size-class filtration and bacterial DNA extraction protocol using commercial "syringe filters", involving sonication- and backflushing-steps and DNA-binding magnetic beads. Importantly, the sonication-step improved DNA recovery up to 10-fold. The DNA extraction-turnaround time is 4h, with a hands-on time of about 2h.

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570 Biowissenschaften, Biologie

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ISO 690KESBERG, Anna Isabella, David SCHLEHECK, 2013. Improved protocol for recovery of bacterial DNA from water filters : Sonication and backflushing of commercial syringe filters. In: Journal of Microbiological Methods. 2013, 93(1), pp. 55-57. ISSN 0167-7012. eISSN 1872-8359. Available under: doi: 10.1016/j.mimet.2013.02.001
BibTex
@article{Kesberg2013-04Impro-22327,
  year={2013},
  doi={10.1016/j.mimet.2013.02.001},
  title={Improved protocol for recovery of bacterial DNA from water filters : Sonication and backflushing of commercial syringe filters},
  number={1},
  volume={93},
  issn={0167-7012},
  journal={Journal of Microbiological Methods},
  pages={55--57},
  author={Kesberg, Anna Isabella and Schleheck, David}
}
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