@article{Schildknecht2006-02-03Autoc-38920,
  year={2006},
  doi={10.1016/j.bbrc.2005.12.009},
  title={Autocatalytic tyrosine nitration of prostaglandin endoperoxide synthase-2 in LPS-stimulated RAW 264.7 macrophages},
  number={1},
  volume={340},
  issn={0006-291X},
  journal={Biochemical and Biophysical Research Communications},
  pages={318--325},
  author={Schildknecht, Stefan and Heinz, Kathrin and Daiber, Andreas and Hamacher, Jürg and Kavaklí, Cengiz and Ullrich, Volker and Bachschmid, Markus}
}

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    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2017-05-16T12:39:42Z</dc:date>
    <dc:contributor>Schildknecht, Stefan</dc:contributor>
    <dc:contributor>Kavaklí, Cengiz</dc:contributor>
    <dc:creator>Daiber, Andreas</dc:creator>
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    <dcterms:title>Autocatalytic tyrosine nitration of prostaglandin endoperoxide synthase-2 in LPS-stimulated RAW 264.7 macrophages</dcterms:title>
    <dcterms:abstract xml:lang="eng">In the literature, biological tyrosine nitrations have been reported to depend not only on peroxynitrite but also on nitrite/hydrogen peroxide linked to catalysis by myeloperoxidase. In endotoxin-stimulated RAW 264.7 macrophages, we have detected a major nitrotyrosine positive protein band around 72 kDa and identified it as prostaglandin endoperoxide synthase-2 (PGHS-2). Isolated PGHS-2 in absence of its substrate arachidonate was not only tyrosine-nitrated with peroxynitrite, but also with nitrite/hydrogen peroxide in complete absence of myeloperoxidase. Our data favor an autocatalytic activation of nitrite by PGHS-2 with a subsequent nitration of the essential tyrosine residue in the cyclooxygenase domain. Under inflammatory conditions, nitrite formed via NO-synthase-2 may therefore act as an endogenous regulator for PGHS-2 in stimulated macrophages. Nitration of PGHS-2 by the autocatalytic activation of nitrite further depends on the intracellular concentration of arachidonate since arachidonate reacted competitively with nitrite and could prevent PGHS-2 from nitration when excessively present.</dcterms:abstract>
    <dc:contributor>Hamacher, Jürg</dc:contributor>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2017-05-16T12:39:42Z</dcterms:available>
    <dc:contributor>Ullrich, Volker</dc:contributor>
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    <dc:creator>Bachschmid, Markus</dc:creator>
    <dc:creator>Hamacher, Jürg</dc:creator>
    <dc:creator>Heinz, Kathrin</dc:creator>
    <dc:contributor>Bachschmid, Markus</dc:contributor>
    <dc:contributor>Daiber, Andreas</dc:contributor>
    <dc:creator>Schildknecht, Stefan</dc:creator>
    <dc:contributor>Heinz, Kathrin</dc:contributor>
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