Publikation:

Autocatalytic tyrosine nitration of prostaglandin endoperoxide synthase-2 in LPS-stimulated RAW 264.7 macrophages

Lade...
Vorschaubild

Dateien

Zu diesem Dokument gibt es keine Dateien.

Datum

2006

Autor:innen

Daiber, Andreas
Hamacher, Jürg
Kavaklí, Cengiz
Bachschmid, Markus

Herausgeber:innen

Kontakt

ISSN der Zeitschrift

Electronic ISSN

ISBN

Bibliografische Daten

Verlag

Schriftenreihe

Auflagebezeichnung

URI (zitierfähiger Link)
ArXiv-ID

Internationale Patentnummer

Angaben zur Forschungsförderung

Projekt

Open Access-Veröffentlichung
Core Facility der Universität Konstanz

Gesperrt bis

Titel in einer weiteren Sprache

Publikationstyp
Zeitschriftenartikel
Publikationsstatus
Published

Erschienen in

Biochemical and Biophysical Research Communications. 2006, 340(1), pp. 318-325. ISSN 0006-291X. eISSN 1090-2104. Available under: doi: 10.1016/j.bbrc.2005.12.009

Zusammenfassung

In the literature, biological tyrosine nitrations have been reported to depend not only on peroxynitrite but also on nitrite/hydrogen peroxide linked to catalysis by myeloperoxidase. In endotoxin-stimulated RAW 264.7 macrophages, we have detected a major nitrotyrosine positive protein band around 72 kDa and identified it as prostaglandin endoperoxide synthase-2 (PGHS-2). Isolated PGHS-2 in absence of its substrate arachidonate was not only tyrosine-nitrated with peroxynitrite, but also with nitrite/hydrogen peroxide in complete absence of myeloperoxidase. Our data favor an autocatalytic activation of nitrite by PGHS-2 with a subsequent nitration of the essential tyrosine residue in the cyclooxygenase domain. Under inflammatory conditions, nitrite formed via NO-synthase-2 may therefore act as an endogenous regulator for PGHS-2 in stimulated macrophages. Nitration of PGHS-2 by the autocatalytic activation of nitrite further depends on the intracellular concentration of arachidonate since arachidonate reacted competitively with nitrite and could prevent PGHS-2 from nitration when excessively present.

Zusammenfassung in einer weiteren Sprache

Fachgebiet (DDC)
570 Biowissenschaften, Biologie

Schlagwörter

Konferenz

Rezension
undefined / . - undefined, undefined

Forschungsvorhaben

Organisationseinheiten

Zeitschriftenheft

Zugehörige Datensätze in KOPS

Zitieren

ISO 690SCHILDKNECHT, Stefan, Kathrin HEINZ, Andreas DAIBER, Jürg HAMACHER, Cengiz KAVAKLÍ, Volker ULLRICH, Markus BACHSCHMID, 2006. Autocatalytic tyrosine nitration of prostaglandin endoperoxide synthase-2 in LPS-stimulated RAW 264.7 macrophages. In: Biochemical and Biophysical Research Communications. 2006, 340(1), pp. 318-325. ISSN 0006-291X. eISSN 1090-2104. Available under: doi: 10.1016/j.bbrc.2005.12.009
BibTex
@article{Schildknecht2006-02-03Autoc-38920,
  year={2006},
  doi={10.1016/j.bbrc.2005.12.009},
  title={Autocatalytic tyrosine nitration of prostaglandin endoperoxide synthase-2 in LPS-stimulated RAW 264.7 macrophages},
  number={1},
  volume={340},
  issn={0006-291X},
  journal={Biochemical and Biophysical Research Communications},
  pages={318--325},
  author={Schildknecht, Stefan and Heinz, Kathrin and Daiber, Andreas and Hamacher, Jürg and Kavaklí, Cengiz and Ullrich, Volker and Bachschmid, Markus}
}
RDF
<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/38920">
    <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/38920"/>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2017-05-16T12:39:42Z</dc:date>
    <dc:contributor>Schildknecht, Stefan</dc:contributor>
    <dc:contributor>Kavaklí, Cengiz</dc:contributor>
    <dc:creator>Daiber, Andreas</dc:creator>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dcterms:title>Autocatalytic tyrosine nitration of prostaglandin endoperoxide synthase-2 in LPS-stimulated RAW 264.7 macrophages</dcterms:title>
    <dcterms:abstract xml:lang="eng">In the literature, biological tyrosine nitrations have been reported to depend not only on peroxynitrite but also on nitrite/hydrogen peroxide linked to catalysis by myeloperoxidase. In endotoxin-stimulated RAW 264.7 macrophages, we have detected a major nitrotyrosine positive protein band around 72 kDa and identified it as prostaglandin endoperoxide synthase-2 (PGHS-2). Isolated PGHS-2 in absence of its substrate arachidonate was not only tyrosine-nitrated with peroxynitrite, but also with nitrite/hydrogen peroxide in complete absence of myeloperoxidase. Our data favor an autocatalytic activation of nitrite by PGHS-2 with a subsequent nitration of the essential tyrosine residue in the cyclooxygenase domain. Under inflammatory conditions, nitrite formed via NO-synthase-2 may therefore act as an endogenous regulator for PGHS-2 in stimulated macrophages. Nitration of PGHS-2 by the autocatalytic activation of nitrite further depends on the intracellular concentration of arachidonate since arachidonate reacted competitively with nitrite and could prevent PGHS-2 from nitration when excessively present.</dcterms:abstract>
    <dc:contributor>Hamacher, Jürg</dc:contributor>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2017-05-16T12:39:42Z</dcterms:available>
    <dc:contributor>Ullrich, Volker</dc:contributor>
    <dcterms:issued>2006-02-03</dcterms:issued>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:creator>Ullrich, Volker</dc:creator>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dc:language>eng</dc:language>
    <dc:creator>Bachschmid, Markus</dc:creator>
    <dc:creator>Hamacher, Jürg</dc:creator>
    <dc:creator>Heinz, Kathrin</dc:creator>
    <dc:contributor>Bachschmid, Markus</dc:contributor>
    <dc:contributor>Daiber, Andreas</dc:contributor>
    <dc:creator>Schildknecht, Stefan</dc:creator>
    <dc:contributor>Heinz, Kathrin</dc:contributor>
    <dc:creator>Kavaklí, Cengiz</dc:creator>
  </rdf:Description>
</rdf:RDF>

Interner Vermerk

xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter

Kontakt
URL der Originalveröffentl.

Prüfdatum der URL

Prüfungsdatum der Dissertation

Finanzierungsart

Kommentar zur Publikation

Allianzlizenz
Corresponding Authors der Uni Konstanz vorhanden
Internationale Co-Autor:innen
Universitätsbibliographie
Nein
Begutachtet
Diese Publikation teilen