Autocatalytic tyrosine nitration of prostaglandin endoperoxide synthase-2 in LPS-stimulated RAW 264.7 macrophages

Lade...
Vorschaubild
Dateien
Zu diesem Dokument gibt es keine Dateien.
Datum
2006
Autor:innen
Daiber, Andreas
Hamacher, Jürg
Kavaklí, Cengiz
Bachschmid, Markus
Herausgeber:innen
Kontakt
ISSN der Zeitschrift
Electronic ISSN
ISBN
Bibliografische Daten
Verlag
Schriftenreihe
Auflagebezeichnung
URI (zitierfähiger Link)
ArXiv-ID
Internationale Patentnummer
Angaben zur Forschungsförderung
Projekt
Open Access-Veröffentlichung
Sammlungen
Core Facility der Universität Konstanz
Gesperrt bis
Titel in einer weiteren Sprache
Forschungsvorhaben
Organisationseinheiten
Zeitschriftenheft
Publikationstyp
Zeitschriftenartikel
Publikationsstatus
Published
Erschienen in
Biochemical and Biophysical Research Communications. 2006, 340(1), pp. 318-325. ISSN 0006-291X. eISSN 1090-2104. Available under: doi: 10.1016/j.bbrc.2005.12.009
Zusammenfassung

In the literature, biological tyrosine nitrations have been reported to depend not only on peroxynitrite but also on nitrite/hydrogen peroxide linked to catalysis by myeloperoxidase. In endotoxin-stimulated RAW 264.7 macrophages, we have detected a major nitrotyrosine positive protein band around 72 kDa and identified it as prostaglandin endoperoxide synthase-2 (PGHS-2). Isolated PGHS-2 in absence of its substrate arachidonate was not only tyrosine-nitrated with peroxynitrite, but also with nitrite/hydrogen peroxide in complete absence of myeloperoxidase. Our data favor an autocatalytic activation of nitrite by PGHS-2 with a subsequent nitration of the essential tyrosine residue in the cyclooxygenase domain. Under inflammatory conditions, nitrite formed via NO-synthase-2 may therefore act as an endogenous regulator for PGHS-2 in stimulated macrophages. Nitration of PGHS-2 by the autocatalytic activation of nitrite further depends on the intracellular concentration of arachidonate since arachidonate reacted competitively with nitrite and could prevent PGHS-2 from nitration when excessively present.

Zusammenfassung in einer weiteren Sprache
Fachgebiet (DDC)
570 Biowissenschaften, Biologie
Schlagwörter
Konferenz
Rezension
undefined / . - undefined, undefined
Zitieren
ISO 690SCHILDKNECHT, Stefan, Kathrin HEINZ, Andreas DAIBER, Jürg HAMACHER, Cengiz KAVAKLÍ, Volker ULLRICH, Markus BACHSCHMID, 2006. Autocatalytic tyrosine nitration of prostaglandin endoperoxide synthase-2 in LPS-stimulated RAW 264.7 macrophages. In: Biochemical and Biophysical Research Communications. 2006, 340(1), pp. 318-325. ISSN 0006-291X. eISSN 1090-2104. Available under: doi: 10.1016/j.bbrc.2005.12.009
BibTex
@article{Schildknecht2006-02-03Autoc-38920,
  year={2006},
  doi={10.1016/j.bbrc.2005.12.009},
  title={Autocatalytic tyrosine nitration of prostaglandin endoperoxide synthase-2 in LPS-stimulated RAW 264.7 macrophages},
  number={1},
  volume={340},
  issn={0006-291X},
  journal={Biochemical and Biophysical Research Communications},
  pages={318--325},
  author={Schildknecht, Stefan and Heinz, Kathrin and Daiber, Andreas and Hamacher, Jürg and Kavaklí, Cengiz and Ullrich, Volker and Bachschmid, Markus}
}
RDF
<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/38920">
    <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/38920"/>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2017-05-16T12:39:42Z</dc:date>
    <dc:contributor>Schildknecht, Stefan</dc:contributor>
    <dc:contributor>Kavaklí, Cengiz</dc:contributor>
    <dc:creator>Daiber, Andreas</dc:creator>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dcterms:title>Autocatalytic tyrosine nitration of prostaglandin endoperoxide synthase-2 in LPS-stimulated RAW 264.7 macrophages</dcterms:title>
    <dcterms:abstract xml:lang="eng">In the literature, biological tyrosine nitrations have been reported to depend not only on peroxynitrite but also on nitrite/hydrogen peroxide linked to catalysis by myeloperoxidase. In endotoxin-stimulated RAW 264.7 macrophages, we have detected a major nitrotyrosine positive protein band around 72 kDa and identified it as prostaglandin endoperoxide synthase-2 (PGHS-2). Isolated PGHS-2 in absence of its substrate arachidonate was not only tyrosine-nitrated with peroxynitrite, but also with nitrite/hydrogen peroxide in complete absence of myeloperoxidase. Our data favor an autocatalytic activation of nitrite by PGHS-2 with a subsequent nitration of the essential tyrosine residue in the cyclooxygenase domain. Under inflammatory conditions, nitrite formed via NO-synthase-2 may therefore act as an endogenous regulator for PGHS-2 in stimulated macrophages. Nitration of PGHS-2 by the autocatalytic activation of nitrite further depends on the intracellular concentration of arachidonate since arachidonate reacted competitively with nitrite and could prevent PGHS-2 from nitration when excessively present.</dcterms:abstract>
    <dc:contributor>Hamacher, Jürg</dc:contributor>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2017-05-16T12:39:42Z</dcterms:available>
    <dc:contributor>Ullrich, Volker</dc:contributor>
    <dcterms:issued>2006-02-03</dcterms:issued>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:creator>Ullrich, Volker</dc:creator>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dc:language>eng</dc:language>
    <dc:creator>Bachschmid, Markus</dc:creator>
    <dc:creator>Hamacher, Jürg</dc:creator>
    <dc:creator>Heinz, Kathrin</dc:creator>
    <dc:contributor>Bachschmid, Markus</dc:contributor>
    <dc:contributor>Daiber, Andreas</dc:contributor>
    <dc:creator>Schildknecht, Stefan</dc:creator>
    <dc:contributor>Heinz, Kathrin</dc:contributor>
    <dc:creator>Kavaklí, Cengiz</dc:creator>
  </rdf:Description>
</rdf:RDF>
Interner Vermerk
xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter
Kontakt
URL der Originalveröffentl.
Prüfdatum der URL
Prüfungsdatum der Dissertation
Finanzierungsart
Kommentar zur Publikation
Allianzlizenz
Corresponding Authors der Uni Konstanz vorhanden
Internationale Co-Autor:innen
Universitätsbibliographie
Nein
Begutachtet
Diese Publikation teilen