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Quantitative analysis of three-dimensional fluorescence localization microscopy data

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Datum

2013

Autor:innen

Owen, Dylan M.
Williamson, David J.
Boelen, Lies
Magenau, Astrid
Gaus, Katharina

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https://doi.org/10.1016/j.bpj.2013.05.063
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Biologie: Publikationen
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Biophysical journal. 2013, 105(2), pp. L05-L07. ISSN 0006-3495. eISSN 1542-0086. Available under: doi: 10.1016/j.bpj.2013.05.063

Zusammenfassung

Identifying the three-dimensional molecular organization of subcellular organelles in intact cells has been challenging to date. Here we present an analysis approach for three-dimensional localization microscopy that can not only identify subcellular objects below the diffraction limit but also quantify their shape and volume. This approach is particularly useful to map the topography of the plasma membrane and measure protein distribution within an undulating membrane.

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570 Biowissenschaften, Biologie

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ISO 690OWEN, Dylan M., David J. WILLIAMSON, Lies BOELEN, Astrid MAGENAU, Jérémie ROSSY, Katharina GAUS, 2013. Quantitative analysis of three-dimensional fluorescence localization microscopy data. In: Biophysical journal. 2013, 105(2), pp. L05-L07. ISSN 0006-3495. eISSN 1542-0086. Available under: doi: 10.1016/j.bpj.2013.05.063
BibTex
@article{Owen2013-07-16Quant-43462,
  year={2013},
  doi={10.1016/j.bpj.2013.05.063},
  title={Quantitative analysis of three-dimensional fluorescence localization microscopy data},
  number={2},
  volume={105},
  issn={0006-3495},
  journal={Biophysical journal},
  pages={L05--L07},
  author={Owen, Dylan M. and Williamson, David J. and Boelen, Lies and Magenau, Astrid and Rossy, Jérémie and Gaus, Katharina}
}
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