Publikation: Phenotype of htgA (mbiA), a recently evolved orphan gene of Escherichia coli and Shigella, completely overlapping in antisense to yaaW
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2014
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Fellner, Lea
Bechtel, Niklas
Witting, Michael A.
Schmitt-Kopplin, Philippe
Scherer, Siegfried
Neuhaus, Klaus
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FEMS Microbiology Letters. 2014, 350(1), pp. 57-64. ISSN 0378-1097. eISSN 1574-6968. Available under: doi: 10.1111/1574-6968.12288
Zusammenfassung
Overlapping embedded genes, such as htgA/yaaW, are assumed to be rare in prokaryotes. In Escherichia coli O157:H7, gfp fusions of both promoter regions revealed activity and transcription start sites could be determined for both genes. Both htgA and yaaW were inactivated strand specifically by introducing a stop codon. Both mutants exhibited differential phenotypes in biofilm formation and metabolite levels in a nontargeted analysis, suggesting that both are functional despite YaaW but not HtgA could be expressed. While yaaW is distributed all over the Gammaproteobacteria, an overlapping htgA-like sequence is restricted to the Escherichia-Klebsiella clade. Full-length htgA is only present in Escherichia and Shigella, and htgA showed evidence for purifying selection. Thus, htgA is an interesting case of a lineage-specific, nonessential and young orphan gene.
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FELLNER, Lea, Niklas BECHTEL, Michael A. WITTING, Svenja SIMON, Philippe SCHMITT-KOPPLIN, Daniel A. KEIM, Siegfried SCHERER, Klaus NEUHAUS, 2014. Phenotype of htgA (mbiA), a recently evolved orphan gene of Escherichia coli and Shigella, completely overlapping in antisense to yaaW. In: FEMS Microbiology Letters. 2014, 350(1), pp. 57-64. ISSN 0378-1097. eISSN 1574-6968. Available under: doi: 10.1111/1574-6968.12288BibTex
@article{Fellner2014-01Pheno-26227,
year={2014},
doi={10.1111/1574-6968.12288},
title={Phenotype of htgA (mbiA), a recently evolved orphan gene of Escherichia coli and Shigella, completely overlapping in antisense to yaaW},
number={1},
volume={350},
issn={0378-1097},
journal={FEMS Microbiology Letters},
pages={57--64},
author={Fellner, Lea and Bechtel, Niklas and Witting, Michael A. and Simon, Svenja and Schmitt-Kopplin, Philippe and Keim, Daniel A. and Scherer, Siegfried and Neuhaus, Klaus}
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<dcterms:abstract xml:lang="eng">Overlapping embedded genes, such as htgA/yaaW, are assumed to be rare in prokaryotes. In Escherichia coli O157:H7, gfp fusions of both promoter regions revealed activity and transcription start sites could be determined for both genes. Both htgA and yaaW were inactivated strand specifically by introducing a stop codon. Both mutants exhibited differential phenotypes in biofilm formation and metabolite levels in a nontargeted analysis, suggesting that both are functional despite YaaW but not HtgA could be expressed. While yaaW is distributed all over the Gammaproteobacteria, an overlapping htgA-like sequence is restricted to the Escherichia-Klebsiella clade. Full-length htgA is only present in Escherichia and Shigella, and htgA showed evidence for purifying selection. Thus, htgA is an interesting case of a lineage-specific, nonessential and young orphan gene.</dcterms:abstract>
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