Publikation: Sequence-Specific Incorporation of Enzyme-Nucleotide Chimera by DNA Polymerases
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Angewandte Chemie International Edition. 2016, 55(34), pp. 10131-10135. ISSN 0570-0833. eISSN 1521-3773. Available under: doi: 10.1002/anie.201604641
Zusammenfassung
DNA polymerases select the right nucleotide for the growing polynucleotide chain based on the shape and geometry of the nascent nucleotide pairs and thereby ensure high DNA replication selectivity. High-fidelity DNA polymerases are believed to possess tight active sites that allow little deviation from the canonical structures. However, DNA polymerases are known to use nucleotides with small modifications as substrates, which is key for numerous core biotechnology applications. We show that even high-fidelity DNA polymerases are capable of efficiently using nucleotide chimera modified with a large protein like horseradish peroxidase as substrates for template-dependent DNA synthesis, despite this "cargo" being more than 100-fold larger than the natural substrates. We exploited this capability for the development of systems that enable naked-eye detection of DNA and RNA at single nucleotide resolution.
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WELTER, Moritz, Daniela VERGA, Andreas MARX, 2016. Sequence-Specific Incorporation of Enzyme-Nucleotide Chimera by DNA Polymerases. In: Angewandte Chemie International Edition. 2016, 55(34), pp. 10131-10135. ISSN 0570-0833. eISSN 1521-3773. Available under: doi: 10.1002/anie.201604641BibTex
@article{Welter2016Seque-36745,
year={2016},
doi={10.1002/anie.201604641},
title={Sequence-Specific Incorporation of Enzyme-Nucleotide Chimera by DNA Polymerases},
number={34},
volume={55},
issn={0570-0833},
journal={Angewandte Chemie International Edition},
pages={10131--10135},
author={Welter, Moritz and Verga, Daniela and Marx, Andreas}
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<dcterms:abstract xml:lang="eng">DNA polymerases select the right nucleotide for the growing polynucleotide chain based on the shape and geometry of the nascent nucleotide pairs and thereby ensure high DNA replication selectivity. High-fidelity DNA polymerases are believed to possess tight active sites that allow little deviation from the canonical structures. However, DNA polymerases are known to use nucleotides with small modifications as substrates, which is key for numerous core biotechnology applications. We show that even high-fidelity DNA polymerases are capable of efficiently using nucleotide chimera modified with a large protein like horseradish peroxidase as substrates for template-dependent DNA synthesis, despite this "cargo" being more than 100-fold larger than the natural substrates. We exploited this capability for the development of systems that enable naked-eye detection of DNA and RNA at single nucleotide resolution.</dcterms:abstract>
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