Publikation: Location of the Active Site and Proposed Catalytic Mechanism of Pterin-4A-Carbinolamine Dehydratase
Dateien
Datum
Autor:innen
Herausgeber:innen
ISSN der Zeitschrift
Electronic ISSN
ISBN
Bibliografische Daten
Verlag
Schriftenreihe
Auflagebezeichnung
URI (zitierfähiger Link)
DOI (zitierfähiger Link)
Internationale Patentnummer
Link zur Lizenz
Angaben zur Forschungsförderung
Projekt
Open Access-Veröffentlichung
Sammlungen
Core Facility der Universität Konstanz
Titel in einer weiteren Sprache
Publikationstyp
Publikationsstatus
Erschienen in
Zusammenfassung
Based on the recently solved three-dimensional structure of pterin-4a-carbinolamine dehydratase from rat/human liver the involvement of the proposed active-site residues Glu57, Asp60, His61, His62, Tyr69, His79, Arg87 and Asp88 was examined by site-directed mutagenesis. Most of the mutants showed reduced activity, and only the Glu57→Ala mutant and the His61→Ala, His62→Ala double mutant were fully devoid of activity. The dissociation constants of quinonoid 6,6-dimethyl-7,8-dihydropterin were significantly increased for binding to the Glu57→Ala, His61→Ala, His62→Ala single mutants and the His61→Ala, His62→Ala double mutant, confirming that His61 and His62 are essential for substrate binding and catalysis. The mechanism of dehydration is proposed to involve base catalysis at the N(5)-H group of the substrate by His61.
Zusammenfassung in einer weiteren Sprache
Fachgebiet (DDC)
Schlagwörter
Konferenz
Rezension
Zitieren
ISO 690
KÖSTER, Sandra, Gunter STIER, Ralf FICNER, Manuela HÖLZER, Hans-Christoph CURTIUS, Dietrich SUCK, Sandro GHISLA, 1996. Location of the Active Site and Proposed Catalytic Mechanism of Pterin-4A-Carbinolamine Dehydratase. In: European Journal of Biochemistry. 1996, 241(3), pp. 858-864. ISSN 0014-2956. eISSN 1432-1033. Available under: doi: 10.1111/j.1432-1033.1996.00858.xBibTex
@article{Koster1996Locat-8396, year={1996}, doi={10.1111/j.1432-1033.1996.00858.x}, title={Location of the Active Site and Proposed Catalytic Mechanism of Pterin-4A-Carbinolamine Dehydratase}, number={3}, volume={241}, issn={0014-2956}, journal={European Journal of Biochemistry}, pages={858--864}, author={Köster, Sandra and Stier, Gunter and Ficner, Ralf and Hölzer, Manuela and Curtius, Hans-Christoph and Suck, Dietrich and Ghisla, Sandro} }
RDF
<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/8396"> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:43:16Z</dc:date> <dc:contributor>Köster, Sandra</dc:contributor> <dc:creator>Hölzer, Manuela</dc:creator> <dc:contributor>Suck, Dietrich</dc:contributor> <dc:contributor>Ghisla, Sandro</dc:contributor> <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/8396/1/Location_of_the_Active_Site_and_Proposed_Catalytic_Mechanism_of_Pterin_4A_Carbinolamine_Dehydratase.pdf"/> <dc:language>eng</dc:language> <dc:contributor>Hölzer, Manuela</dc:contributor> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:43:16Z</dcterms:available> <dc:creator>Suck, Dietrich</dc:creator> <dcterms:rights rdf:resource="http://creativecommons.org/licenses/by-nc-nd/2.0/"/> <foaf:homepage rdf:resource="http://localhost:8080/"/> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dc:contributor>Ficner, Ralf</dc:contributor> <dc:contributor>Curtius, Hans-Christoph</dc:contributor> <dcterms:bibliographicCitation>First publ. in: European Journal of Biochemistry 241 (1996), 3, pp. 858-864</dcterms:bibliographicCitation> <dcterms:abstract xml:lang="eng">Based on the recently solved three-dimensional structure of pterin-4a-carbinolamine dehydratase from rat/human liver the involvement of the proposed active-site residues Glu57, Asp60, His61, His62, Tyr69, His79, Arg87 and Asp88 was examined by site-directed mutagenesis. Most of the mutants showed reduced activity, and only the Glu57→Ala mutant and the His61→Ala, His62→Ala double mutant were fully devoid of activity. The dissociation constants of quinonoid 6,6-dimethyl-7,8-dihydropterin were significantly increased for binding to the Glu57→Ala, His61→Ala, His62→Ala single mutants and the His61→Ala, His62→Ala double mutant, confirming that His61 and His62 are essential for substrate binding and catalysis. The mechanism of dehydration is proposed to involve base catalysis at the N(5)-H group of the substrate by His61.</dcterms:abstract> <dc:rights>Attribution-NonCommercial-NoDerivs 2.0 Generic</dc:rights> <dcterms:title>Location of the Active Site and Proposed Catalytic Mechanism of Pterin-4A-Carbinolamine Dehydratase</dcterms:title> <dcterms:issued>1996</dcterms:issued> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/8396"/> <dc:contributor>Stier, Gunter</dc:contributor> <dc:creator>Köster, Sandra</dc:creator> <dc:format>application/pdf</dc:format> <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/8396/1/Location_of_the_Active_Site_and_Proposed_Catalytic_Mechanism_of_Pterin_4A_Carbinolamine_Dehydratase.pdf"/> <dc:creator>Ficner, Ralf</dc:creator> <dc:creator>Curtius, Hans-Christoph</dc:creator> <dc:creator>Stier, Gunter</dc:creator> <dc:creator>Ghisla, Sandro</dc:creator> </rdf:Description> </rdf:RDF>