Identification of Bilophila wadsworthia by specific PCR which targets the taurine:pyruvate aminotransferase gene

Kurzanzeige
dc.contributor.authorLaue, Heikedeu
dc.contributor.authorSmits, Theo H. M.deu
dc.contributor.authorSchumacher, Ulrike K.deu
dc.contributor.authorClaros, Marina C.deu
dc.contributor.authorHartemink, Ralfdeu
dc.contributor.authorCook, Alasdair M.
dc.date.accessioned2011-03-24T17:33:57Zdeu
dc.date.available2011-03-24T17:33:57Zdeu
dc.date.issued2006deu
dc.description.abstractThe bile-resistant, strictly anaerobic bacterium Bilophila wadsworthia is found in human faecal flora, in human infections and in environmental samples. A specific PCR primer set for the gene encoding the first metabolic enzyme in the degradative pathway for taurine in B. wadsworthia, taurine:pyruvate aminotransferase (tpa), was developed and tested. In addition, enrichment cultures were started from faecal samples of primates and felines and shown to contain B. wadsworthia. These were subcultured on agar media and then identified by PCR fingerprinting. PCR for tpa was successful in all positive enrichment cultures and showed no amplification signal in a variety of other bacterial species. Therefore, this PCR method could be a promising tool for rapid detection of B. wadsworthia in biological samples.eng
dc.description.versionpublished
dc.format.mimetypeapplication/pdfdeu
dc.identifier.citationFirst publ. in: FEMS Microbiology Letters 261 (2006), 1, pp. 74 - 79deu
dc.identifier.doi10.1111/j.1574-6968.2006.00335.x
dc.identifier.pmid16842362
dc.identifier.ppn288165802deu
dc.identifier.urihttp://kops.uni-konstanz.de/handle/123456789/7380
dc.language.isoengdeu
dc.legacy.dateIssued2008deu
dc.rightsAttribution-NonCommercial-NoDerivs 2.0 Generic
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/2.0/
dc.subjectBilophila wadsworthia isolationdeu
dc.subjecttaurine dissimilationdeu
dc.subjecttaurine:pyruvate aminotransferasedeu
dc.subject.ddc570deu
dc.titleIdentification of Bilophila wadsworthia by specific PCR which targets the taurine:pyruvate aminotransferase geneeng
dc.typeJOURNAL_ARTICLEdeu
dspace.entity.typePublication
kops.citation.bibtex
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  year={2006},
  doi={10.1111/j.1574-6968.2006.00335.x},
  title={Identification of Bilophila wadsworthia by specific PCR which targets the taurine:pyruvate aminotransferase gene},
  number={1},
  volume={261},
  issn={0378-1097},
  journal={FEMS Microbiology Letters},
  pages={74--79},
  author={Laue, Heike and Smits, Theo H. M. and Schumacher, Ulrike K. and Claros, Marina C. and Hartemink, Ralf and Cook, Alasdair M.}
}
kops.citation.iso690LAUE, Heike, Theo H. M. SMITS, Ulrike K. SCHUMACHER, Marina C. CLAROS, Ralf HARTEMINK, Alasdair M. COOK, 2006. Identification of Bilophila wadsworthia by specific PCR which targets the taurine:pyruvate aminotransferase gene. In: FEMS Microbiology Letters. 2006, 261(1), pp. 74-79. ISSN 0378-1097. eISSN 1574-6968. Available under: doi: 10.1111/j.1574-6968.2006.00335.xdeu
kops.citation.iso690LAUE, Heike, Theo H. M. SMITS, Ulrike K. SCHUMACHER, Marina C. CLAROS, Ralf HARTEMINK, Alasdair M. COOK, 2006. Identification of Bilophila wadsworthia by specific PCR which targets the taurine:pyruvate aminotransferase gene. In: FEMS Microbiology Letters. 2006, 261(1), pp. 74-79. ISSN 0378-1097. eISSN 1574-6968. Available under: doi: 10.1111/j.1574-6968.2006.00335.xeng
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kops.sourcefieldFEMS Microbiology Letters. 2006, <b>261</b>(1), pp. 74-79. ISSN 0378-1097. eISSN 1574-6968. Available under: doi: 10.1111/j.1574-6968.2006.00335.xdeu
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