Publikation:

Identification of Bilophila wadsworthia by specific PCR which targets the taurine:pyruvate aminotransferase gene

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Datum

2006

Autor:innen

Laue, Heike
Smits, Theo H. M.
Schumacher, Ulrike K.
Claros, Marina C.
Hartemink, Ralf

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http://nbn-resolving.de/urn:nbn:de:bsz:352-opus-67364
DOI (zitierfähiger Link)
https://doi.org/10.1111/j.1574-6968.2006.00335.x
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Attribution-NonCommercial-NoDerivs 2.0 Generic

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Open Access Green

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Published

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FEMS Microbiology Letters. 2006, 261(1), pp. 74-79. ISSN 0378-1097. eISSN 1574-6968. Available under: doi: 10.1111/j.1574-6968.2006.00335.x

Zusammenfassung

The bile-resistant, strictly anaerobic bacterium Bilophila wadsworthia is found in human faecal flora, in human infections and in environmental samples. A specific PCR primer set for the gene encoding the first metabolic enzyme in the degradative pathway for taurine in B. wadsworthia, taurine:pyruvate aminotransferase (tpa), was developed and tested. In addition, enrichment cultures were started from faecal samples of primates and felines and shown to contain B. wadsworthia. These were subcultured on agar media and then identified by PCR fingerprinting. PCR for tpa was successful in all positive enrichment cultures and showed no amplification signal in a variety of other bacterial species. Therefore, this PCR method could be a promising tool for rapid detection of B. wadsworthia in biological samples.

Zusammenfassung in einer weiteren Sprache

Fachgebiet (DDC)
570 Biowissenschaften, Biologie

Schlagwörter

Bilophila wadsworthia isolation, taurine dissimilation, taurine:pyruvate aminotransferase

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ISO 690LAUE, Heike, Theo H. M. SMITS, Ulrike K. SCHUMACHER, Marina C. CLAROS, Ralf HARTEMINK, Alasdair M. COOK, 2006. Identification of Bilophila wadsworthia by specific PCR which targets the taurine:pyruvate aminotransferase gene. In: FEMS Microbiology Letters. 2006, 261(1), pp. 74-79. ISSN 0378-1097. eISSN 1574-6968. Available under: doi: 10.1111/j.1574-6968.2006.00335.x
BibTex
@article{Laue2006Ident-7380,
  year={2006},
  doi={10.1111/j.1574-6968.2006.00335.x},
  title={Identification of Bilophila wadsworthia by specific PCR which targets the taurine:pyruvate aminotransferase gene},
  number={1},
  volume={261},
  issn={0378-1097},
  journal={FEMS Microbiology Letters},
  pages={74--79},
  author={Laue, Heike and Smits, Theo H. M. and Schumacher, Ulrike K. and Claros, Marina C. and Hartemink, Ralf and Cook, Alasdair M.}
}
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