Publikation: A dual-purpose polymerase engineered for direct sequencing of pseudouridine and queuosine
Dateien
Datum
Autor:innen
Herausgeber:innen
ISSN der Zeitschrift
Electronic ISSN
ISBN
Bibliografische Daten
Verlag
Schriftenreihe
Auflagebezeichnung
URI (zitierfähiger Link)
DOI (zitierfähiger Link)
Internationale Patentnummer
Link zur Lizenz
Angaben zur Forschungsförderung
Projekt
Open Access-Veröffentlichung
Sammlungen
Core Facility der Universität Konstanz
Titel in einer weiteren Sprache
Publikationstyp
Publikationsstatus
Erschienen in
Zusammenfassung
More than 170 posttranscriptional RNA modifications are so far known on both coding and noncoding RNA species. Within this group, pseudouridine (Ψ) and queuosine (Q) represent conserved RNA modifications with fundamental functional roles in regulating translation. Current detection methods of these modifications, which both are reverse transcription (RT)-silent, are mostly based on the chemical treatment of RNA prior to analysis. To overcome the drawbacks associated with indirect detection strategies, we have engineered an RT-active DNA polymerase variant called RT-KTq I614Y that produces error RT signatures specific for Ψ or Q without prior chemical treatment of the RNA samples. Combining this polymerase with next-generation sequencing techniques allows the direct identification of Ψ and Q sites of untreated RNA samples using a single enzymatic tool.
Zusammenfassung in einer weiteren Sprache
Fachgebiet (DDC)
Schlagwörter
Konferenz
Rezension
Zitieren
ISO 690
HUBER, Luisa B., Navpreet KAUR, Melanie HENKEL, Virginie MARCHAND, Yuri MOTORIN, Ann E EHRENHOFER-MURRAY, Andreas MARX, 2023. A dual-purpose polymerase engineered for direct sequencing of pseudouridine and queuosine. In: Nucleic Acids Research. Oxford University Press. 2023, 51(8), pp. 3971-3987. ISSN 0305-1048. eISSN 1362-4962. Available under: doi: 10.1093/nar/gkad177BibTex
@article{Huber2023-03-27dualp-66545, year={2023}, doi={10.1093/nar/gkad177}, title={A dual-purpose polymerase engineered for direct sequencing of pseudouridine and queuosine}, number={8}, volume={51}, issn={0305-1048}, journal={Nucleic Acids Research}, pages={3971--3987}, author={Huber, Luisa B. and Kaur, Navpreet and Henkel, Melanie and Marchand, Virginie and Motorin, Yuri and Ehrenhofer-Murray, Ann E and Marx, Andreas} }
RDF
<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/66545"> <dcterms:issued>2023-03-27</dcterms:issued> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/29"/> <dcterms:rights rdf:resource="http://creativecommons.org/licenses/by-nc/4.0/"/> <dc:contributor>Kaur, Navpreet</dc:contributor> <dc:contributor>Henkel, Melanie</dc:contributor> <dc:creator>Kaur, Navpreet</dc:creator> <dc:contributor>Huber, Luisa B.</dc:contributor> <dc:contributor>Motorin, Yuri</dc:contributor> <foaf:homepage rdf:resource="http://localhost:8080/"/> <dc:creator>Henkel, Melanie</dc:creator> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/29"/> <dc:contributor>Marx, Andreas</dc:contributor> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2023-04-06T11:30:33Z</dcterms:available> <dc:contributor>Ehrenhofer-Murray, Ann E</dc:contributor> <dc:creator>Motorin, Yuri</dc:creator> <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/66545/1/Huber_2-159qzwymdocna3.pdf"/> <dc:creator>Marchand, Virginie</dc:creator> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2023-04-06T11:30:33Z</dc:date> <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/66545"/> <dcterms:abstract>More than 170 posttranscriptional RNA modifications are so far known on both coding and noncoding RNA species. Within this group, pseudouridine (Ψ) and queuosine (Q) represent conserved RNA modifications with fundamental functional roles in regulating translation. Current detection methods of these modifications, which both are reverse transcription (RT)-silent, are mostly based on the chemical treatment of RNA prior to analysis. To overcome the drawbacks associated with indirect detection strategies, we have engineered an RT-active DNA polymerase variant called RT-KTq I614Y that produces error RT signatures specific for Ψ or Q without prior chemical treatment of the RNA samples. Combining this polymerase with next-generation sequencing techniques allows the direct identification of Ψ and Q sites of untreated RNA samples using a single enzymatic tool.</dcterms:abstract> <dc:language>eng</dc:language> <dc:rights>Attribution-NonCommercial 4.0 International</dc:rights> <dc:contributor>Marchand, Virginie</dc:contributor> <dc:creator>Ehrenhofer-Murray, Ann E</dc:creator> <dc:creator>Marx, Andreas</dc:creator> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/66545/1/Huber_2-159qzwymdocna3.pdf"/> <dc:creator>Huber, Luisa B.</dc:creator> <dcterms:title>A dual-purpose polymerase engineered for direct sequencing of pseudouridine and queuosine</dcterms:title> </rdf:Description> </rdf:RDF>