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Transient Expression of ESCRT Components in Arabidopsis Root Cell Suspension Culture-Derived Protoplasts

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2019

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https://doi.org/10.1007/978-1-4939-9492-2_12
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CULETTO, Emmanuel, ed., Renaud LEGOUIS, ed.. The ESCRT Complexes : Methods and Protocols. New York, NY: Humana, 2019, pp. 163-174. Methods in Molecular Biology. 1998. ISBN 978-1-4939-9491-5. Available under: doi: 10.1007/978-1-4939-9492-2_12

Zusammenfassung

Localization studies are important to understand the function of diverse proteins. The endosomal trafficking pathway is very complex, and a lot of proteins function in this pathway, primarily the endosomal sorting complexes required for transport (ESCRTs). Some of the ESCRT-related proteins or mutant variants cannot be stably expressed in planta due to the toxicity of their expression. Therefore, a transient expression system is necessary to study their function. Transient expression in protoplasts from Arabidopsis root cell-derived culture serves as a fast and reliable method for the expression and cell biological and biochemical analyses of otherwise toxic constructs.

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570 Biowissenschaften, Biologie

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Transient expression, Protoplast, ESCRT, Microscopy, Arabidopsis cell culture

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ISO 690NAGEL, Marie-Kristin, Karin VOGEL, Erika ISONO, 2019. Transient Expression of ESCRT Components in Arabidopsis Root Cell Suspension Culture-Derived Protoplasts. In: CULETTO, Emmanuel, ed., Renaud LEGOUIS, ed.. The ESCRT Complexes : Methods and Protocols. New York, NY: Humana, 2019, pp. 163-174. Methods in Molecular Biology. 1998. ISBN 978-1-4939-9491-5. Available under: doi: 10.1007/978-1-4939-9492-2_12
BibTex
@incollection{Nagel2019Trans-47162,
  year={2019},
  doi={10.1007/978-1-4939-9492-2_12},
  title={Transient Expression of ESCRT Components in Arabidopsis Root Cell Suspension Culture-Derived Protoplasts},
  number={1998},
  isbn={978-1-4939-9491-5},
  publisher={Humana},
  address={New York, NY},
  series={Methods in Molecular Biology},
  booktitle={The ESCRT Complexes : Methods and Protocols},
  pages={163--174},
  editor={Culetto, Emmanuel and Legouis, Renaud},
  author={Nagel, Marie-Kristin and Vogel, Karin and Isono, Erika}
}
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