Publikation:

A flexible and fully integrated system for amplification, detection and genotyping of genomic DNA targets based on microfluidic oligonucleotide arrays

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2010

Autor:innen

Summerer, Daniel
Hevroni, Dona
Jain, Amit
Oldenburger, Olga
Parker, Jefferson
Caruso, Anthony
Stähler, Cord F.
Stähler, Peer F.
Beier, Markus

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http://nbn-resolving.de/urn:nbn:de:bsz:352-opus-128242
DOI (zitierfähiger Link)
https://doi.org/10.1016/j.nbt.2010.03.005
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Chemie: Publikationen
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Published

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New Biotechnology. 2010, 27(2), pp. 149-155. ISSN 1871-6784. eISSN 1876-4347. Available under: doi: 10.1016/j.nbt.2010.03.005

Zusammenfassung

A strategy allowing for amplification, detection and genotyping of different genomic DNA targets in a single reaction container is described. The method makes use of primer-directed solution-phase amplification with integrated labeling in a closed, microfluidic oligonucleotide array. Selective array probes allow for subsequent detection and genotyping of generated amplicons by hybridization. The array contains up to 15,624 programmable features that can be designed, de novo synthesized and tested within 24 hours using an automated benchtop microarray synthesizer. This enables rapid prototyping and adaptation of the system to newly emerging targets such as pathogenic bacterial or viral subtypes. The system was evaluated by amplifying and detecting different loci of viral (HPV), bacterial (Bacillus sp.) and eukaryotic (human) genomes. Multiplex PCR and semi-quantitative detection with excellent detection limits of

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540 Chemie

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ISO 690SUMMERER, Daniel, Dona HEVRONI, Amit JAIN, Olga OLDENBURGER, Jefferson PARKER, Anthony CARUSO, Cord F. STÄHLER, Peer F. STÄHLER, Markus BEIER, 2010. A flexible and fully integrated system for amplification, detection and genotyping of genomic DNA targets based on microfluidic oligonucleotide arrays. In: New Biotechnology. 2010, 27(2), pp. 149-155. ISSN 1871-6784. eISSN 1876-4347. Available under: doi: 10.1016/j.nbt.2010.03.005
BibTex
@article{Summerer2010flexi-9539,
  year={2010},
  doi={10.1016/j.nbt.2010.03.005},
  title={A flexible and fully integrated system for amplification, detection and genotyping of genomic DNA targets based on microfluidic oligonucleotide arrays},
  number={2},
  volume={27},
  issn={1871-6784},
  journal={New Biotechnology},
  pages={149--155},
  author={Summerer, Daniel and Hevroni, Dona and Jain, Amit and Oldenburger, Olga and Parker, Jefferson and Caruso, Anthony and Stähler, Cord F. and Stähler, Peer F. and Beier, Markus}
}
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