Publikation: Endoplasmatic Reticulum subcompartments in a plant parasitic fungus and in Baker's Yeast : differential distribution of lumenal proteins
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His-Asp-Glu-Leu (HDEL)-bearing proteins were quantified in different endoplasmic reticulum (ER) subcompartments of Saccharomyces cerevisiae and the plant parasite Uromyces viciae-fabae by immuno-electron microscopy (immuno-EM). In both fungi, the immunogold labeling of these proteins within the ER was three times greater than within the nuclear envelope. In U.viciae-fabae, the ER in germinating uredospores differed from the ER in fungal stuctures produced within the plant, e.g., haustoria. In haustoria, the cisternal ER differentiated large tubula-vesicular complexes (TVC). TVC contained higher levels of HDEL-bearing proteins than ordinary ER cisternae. ELISA readings also indicated an increased concentration of these proteins in isolated haustoria compared to germinating uredospores. Our results suggest that ER subcompartments can be differentiated by immunogold labeling of proteins with a retention signal. In special cases, such as in the parasitic phase of rust fungi, these proteins accumulate to higher levels in ER subcompartments, probably as a response to plant-induced stress.
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BACHEM, Ulrich, Kurt MENDGEN, 1995. Endoplasmatic Reticulum subcompartments in a plant parasitic fungus and in Baker's Yeast : differential distribution of lumenal proteins. In: Experimental Mycology. 1995, 19, pp. 137-152BibTex
@article{Bachem1995Endop-6613, year={1995}, title={Endoplasmatic Reticulum subcompartments in a plant parasitic fungus and in Baker's Yeast : differential distribution of lumenal proteins}, volume={19}, journal={Experimental Mycology}, pages={137--152}, author={Bachem, Ulrich and Mendgen, Kurt} }
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