Publikation:

The role of C4 metabolism in the marine diatom Phaeodactylum tricornutum

Lade...
Vorschaubild

Dateien

Haimovich_219675.pdf
Haimovich_219675.pdfGröße: 5.53 MBDownloads: 941

Datum

2013

Autor:innen

Haimovich-Dayan, Maya
Garfinkel, Nitsan
Marcus, Yehouda
Wagner, Heiko
Kaplan, Aaron

Herausgeber:innen

Kontakt

ISSN der Zeitschrift

Electronic ISSN

ISBN

Bibliografische Daten

Verlag

Schriftenreihe

Auflagebezeichnung

ArXiv-ID

Internationale Patentnummer

Angaben zur Forschungsförderung

Projekt

Living well with a scrambled metabolism
Open Access-Veröffentlichung
Open Access Green
Core Facility der Universität Konstanz

Gesperrt bis

Titel in einer weiteren Sprache

Publikationstyp
Zeitschriftenartikel
Publikationsstatus
Published

Erschienen in

New Phytologist. 2013, 197(1), pp. 177-185. ISSN 0028-646X. eISSN 1469-8137. Available under: doi: 10.1111/j.1469-8137.2012.04375.x

Zusammenfassung

Diatoms are important players in the global carbon cycle. Their apparent photosynthetic affinity for ambient CO(2) is much higher than that of ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco), indicating that a CO(2)-concentrating mechanism (CCM) is functioning. However, the nature of the CCM, a biophysical or a biochemical C(4), remains elusive. Although (14)C labeling experiments and presence of complete sets of genes for C(4) metabolism in two diatoms supported the presence of C(4), other data and predicted localization of the decarboxylating enzymes, away from Rubisco, makes this unlikely. We used RNA-interference to silence the single gene encoding pyruvate-orthophosphate dikinase (PPDK) in Phaeodactylum tricornutum, essential for C(4) metabolism, and examined the photosynthetic characteristics. The mutants possess much lower ppdk transcript and PPDK activity but the photosynthetic K(1/2) (CO(2)) was hardly affected, thus clearly indicating that the C(4) route does not serve the purpose of raising the CO(2) concentration in close proximity of Rubisco in P. tricornutum. The photosynthetic V(max) was slightly reduced in the mutant, possibly reflecting a metabolic constraint that also resulted in a larger lipid accumulation. We propose that the C(4) metabolism does not function in net CO(2) fixation but helps the cells to dissipate excess light energy and in pH homeostasis.

Zusammenfassung in einer weiteren Sprache

Fachgebiet (DDC)
570 Biowissenschaften, Biologie

Schlagwörter

Konferenz

Rezension
undefined / . - undefined, undefined

Forschungsvorhaben

Organisationseinheiten

Zeitschriftenheft

Zugehörige Datensätze in KOPS

Zitieren

ISO 690HAIMOVICH-DAYAN, Maya, Nitsan GARFINKEL, Daniela EWE, Yehouda MARCUS, Ansgar GRUBER, Heiko WAGNER, Peter G. KROTH, Aaron KAPLAN, 2013. The role of C4 metabolism in the marine diatom Phaeodactylum tricornutum. In: New Phytologist. 2013, 197(1), pp. 177-185. ISSN 0028-646X. eISSN 1469-8137. Available under: doi: 10.1111/j.1469-8137.2012.04375.x
BibTex
@article{HaimovichDayan2013-01metab-21967,
  year={2013},
  doi={10.1111/j.1469-8137.2012.04375.x},
  title={The role of C<sub>4</sub> metabolism in the marine diatom Phaeodactylum tricornutum},
  number={1},
  volume={197},
  issn={0028-646X},
  journal={New Phytologist},
  pages={177--185},
  author={Haimovich-Dayan, Maya and Garfinkel, Nitsan and Ewe, Daniela and Marcus, Yehouda and Gruber, Ansgar and Wagner, Heiko and Kroth, Peter G. and Kaplan, Aaron}
}
RDF
<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/21967">
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:rights>terms-of-use</dc:rights>
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/21967/2/Haimovich_219675.pdf"/>
    <dc:contributor>Haimovich-Dayan, Maya</dc:contributor>
    <dc:creator>Kroth, Peter G.</dc:creator>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dc:creator>Garfinkel, Nitsan</dc:creator>
    <dc:contributor>Garfinkel, Nitsan</dc:contributor>
    <dc:contributor>Gruber, Ansgar</dc:contributor>
    <dc:creator>Gruber, Ansgar</dc:creator>
    <dc:creator>Kaplan, Aaron</dc:creator>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2013-02-20T10:52:32Z</dcterms:available>
    <dc:contributor>Ewe, Daniela</dc:contributor>
    <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/>
    <dcterms:issued>2013-01</dcterms:issued>
    <dc:creator>Haimovich-Dayan, Maya</dc:creator>
    <dcterms:title>The role of C&lt;sub&gt;4&lt;/sub&gt; metabolism in the marine diatom Phaeodactylum tricornutum</dcterms:title>
    <dc:language>eng</dc:language>
    <dc:contributor>Kaplan, Aaron</dc:contributor>
    <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/21967"/>
    <dc:contributor>Kroth, Peter G.</dc:contributor>
    <dcterms:abstract>Diatoms are important players in the global carbon cycle. Their apparent photosynthetic affinity for ambient CO(2) is much higher than that of ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco), indicating that a CO(2)-concentrating mechanism (CCM) is functioning. However, the nature of the CCM, a biophysical or a biochemical C(4), remains elusive. Although (14)C labeling experiments and presence of complete sets of genes for C(4) metabolism in two diatoms supported the presence of C(4), other data and predicted localization of the decarboxylating enzymes, away from Rubisco, makes this unlikely. We used RNA-interference to silence the single gene encoding pyruvate-orthophosphate dikinase (PPDK) in Phaeodactylum tricornutum, essential for C(4) metabolism, and examined the photosynthetic characteristics. The mutants possess much lower ppdk transcript and PPDK activity but the photosynthetic K(1/2) (CO(2)) was hardly affected, thus clearly indicating that the C(4) route does not serve the purpose of raising the CO(2) concentration in close proximity of Rubisco in P. tricornutum. The photosynthetic V(max) was slightly reduced in the mutant, possibly reflecting a metabolic constraint that also resulted in a larger lipid accumulation. We propose that the C(4) metabolism does not function in net CO(2) fixation but helps the cells to dissipate excess light energy and in pH homeostasis.</dcterms:abstract>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dc:contributor>Marcus, Yehouda</dc:contributor>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:creator>Marcus, Yehouda</dc:creator>
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/21967/2/Haimovich_219675.pdf"/>
    <dcterms:bibliographicCitation>New Phytologist ; 197 (2013), 1. - S. 177-185</dcterms:bibliographicCitation>
    <dc:creator>Wagner, Heiko</dc:creator>
    <dc:creator>Ewe, Daniela</dc:creator>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2013-02-20T10:52:32Z</dc:date>
    <dc:contributor>Wagner, Heiko</dc:contributor>
  </rdf:Description>
</rdf:RDF>

Interner Vermerk

xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter

Kontakt
URL der Originalveröffentl.

Prüfdatum der URL

Prüfungsdatum der Dissertation

Finanzierungsart

Kommentar zur Publikation

Allianzlizenz
Corresponding Authors der Uni Konstanz vorhanden
Internationale Co-Autor:innen
Universitätsbibliographie
Ja
Begutachtet
Diese Publikation teilen