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Pulsed EPR spectroscopy distance measurements of DNA internally labelled with Gd3+-DOTA

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2015

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Chemical communications. 2015, 51(72), pp. 13850-13853. ISSN 0009-241X. eISSN 1364-548X. Available under: doi: 10.1039/c5cc04234h

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Gd3+ is increasingly used in EPR spectroscopy due to its increased intracellular stability and signal-to-noise ratios. Here we present the incorporation of Gd3+-DOTA into internal positions in DNA. Distance measurements via pulsed Electron Paramagnetic Resonance (EPR) spectroscopy in vitro and in cellula proved enhanced stability and efficiency compared to nitroxide labels.

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540 Chemie

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ISO 690WOJCIECHOWSKI, Filip, Andreas GROSS, Isabelle T. HOLDER, Laura JOHN, Malte DRESCHER, Jörg S. HARTIG, 2015. Pulsed EPR spectroscopy distance measurements of DNA internally labelled with Gd3+-DOTA. In: Chemical communications. 2015, 51(72), pp. 13850-13853. ISSN 0009-241X. eISSN 1364-548X. Available under: doi: 10.1039/c5cc04234h
BibTex
@article{Wojciechowski2015Pulse-33144,
  year={2015},
  doi={10.1039/c5cc04234h},
  title={Pulsed EPR spectroscopy distance measurements of DNA internally labelled with Gd<sup>3+</sup>-DOTA},
  number={72},
  volume={51},
  issn={0009-241X},
  journal={Chemical communications},
  pages={13850--13853},
  author={Wojciechowski, Filip and Groß, Andreas and Holder, Isabelle T. and John, Laura and Drescher, Malte and Hartig, Jörg S.}
}
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