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Simian‐virus‐40 large‐T‐antigen‐catalyzed DNA and RNA unwinding reactions

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1991

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European Journal of Biochemistry. 1991, 195(1), pp. 49-54. ISSN 0014-2956. eISSN 1432-1033. Available under: doi: 10.1111/j.1432-1033.1991.tb15674.x

Zusammenfassung

Simian virus 40 large T antigen is a helicase separating the complementary strands of double-stranded DNA in the presence of hydrolyzable ATP and of double-stranded RNA in the presence of non-ATP nucleotides (GTP, CTP or UTP). We have constructed partially single-stranded nucleic acid substrates consisting of RNA or DNA strands hydrogen bonded to either RNA or DNA complements. We found that ATP is utilized as a cofactor for the T-antigen-catalyzed unwinding reaction when the substrates contain overhanging single-stranded DNA, regardless of whether the double-stranded region is DNA or hybrid DNA · RNA. Conversely, non-ATP nucleotides are used when the overhanging single strand is RNA. Based on these and additional findings, we propose that the bound nucleic acid induces a conformational change in T antigen resulting in a proper orientation of both nucleic acid and nucleotide relative to the active center of the ATPase/helicase domain of the enzyme. The implications of our conclusion for the roles which T antigen may play in vivo are discussed.

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570 Biowissenschaften, Biologie

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ISO 690SCHEFFNER, Martin, Rolf KNIPPERS, Hans STAHL, 1991. Simian‐virus‐40 large‐T‐antigen‐catalyzed DNA and RNA unwinding reactions. In: European Journal of Biochemistry. 1991, 195(1), pp. 49-54. ISSN 0014-2956. eISSN 1432-1033. Available under: doi: 10.1111/j.1432-1033.1991.tb15674.x
BibTex
@article{Scheffner1991Simia-42732,
  year={1991},
  doi={10.1111/j.1432-1033.1991.tb15674.x},
  title={Simian‐virus‐40 large‐T‐antigen‐catalyzed DNA and RNA unwinding reactions},
  number={1},
  volume={195},
  issn={0014-2956},
  journal={European Journal of Biochemistry},
  pages={49--54},
  author={Scheffner, Martin and Knippers, Rolf and Stahl, Hans}
}
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