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Monitoring E. amylovora using Real Time PCR

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2010

Autor:innen

Olbrecht, L.
Hinze, Malin
Weißhaupt, S.
Schmid, A.
Ernst, M.
Joos, M.
Matschinsky, M.

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Beitrag zu einem Konferenzband
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Published

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FÖRDERGEMEINSCHAFT ÖKOLOGISCHER OBSTBAU E. V., , ed.. Eco-Fruit : proceedings to the conference from February 22 nd to February 24 th, 2010 at the University of Hohenheim, Germany ; 14 th International Conference on Organic Fruit-Growing. Weinsberg: Fördergemeinschaft Ökologischer Obstbau, 2010, pp. 110-117. ISBN 978-3-00-030214-5

Zusammenfassung

Fire blight, caused by the Gram-negative bacterium Erwinia amylovora is the most damaging bacterial disease of pome fruit world wide. Disease outbreak is only sporadic, disease outcome, however, can be devastating. Aside from physical factors like temperature and humidity, presence of the pathogen in sufficient amounts to cause infection is a crucial aspect in abatement of disease. We have refined a real time PCR based quantification of E. amylovora to be used in the field on a variety of environmental samples. This method allowed us to monitor pathogen abundance during blossom during the last four years. Integration of the amount of pathogen detected into refined prediction algorithms will most certainly improve applied forecasting models. The method also allows the quantification of pathogen outside the blooming period within or on the surface of different tissue, or plant organs. This may allow a monitoring of the pathogen population throughout the year and in the long run may facilitate timely countermeasures. Results also indicate a potential role of fruit mummies in overwintering and dissemination of the pathogen early in the growing season, as well as a role of non-host flowers in the built up of pathogen potential. The method is also capable of monitoring disease progression in infected, but symptomless tissue. Results from this set of experiments might improve recommendations for good cultural practice.

Zusammenfassung in einer weiteren Sprache

Fachgebiet (DDC)
570 Biowissenschaften, Biologie

Schlagwörter

E. amylovora, epidemiology, monitoring, pathogenesis, Real Time PCR

Konferenz

Eco-Fruit, 22. Feb. 2010 - 24. Feb. 2010, University of Hohenheim, Germany
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ISO 690VOEGELE, Ralf T., Stefan KUNZ, L. OLBRECHT, Malin HINZE, S. WEISSHAUPT, A. SCHMID, M. ERNST, M. JOOS, M. MATSCHINSKY, Kurt MENDGEN, 2010. Monitoring E. amylovora using Real Time PCR. Eco-Fruit. University of Hohenheim, Germany, 22. Feb. 2010 - 24. Feb. 2010. In: FÖRDERGEMEINSCHAFT ÖKOLOGISCHER OBSTBAU E. V., , ed.. Eco-Fruit : proceedings to the conference from February 22 nd to February 24 th, 2010 at the University of Hohenheim, Germany ; 14 th International Conference on Organic Fruit-Growing. Weinsberg: Fördergemeinschaft Ökologischer Obstbau, 2010, pp. 110-117. ISBN 978-3-00-030214-5
BibTex
@inproceedings{Voegele2010Monit-15162,
  year={2010},
  title={Monitoring E. amylovora using Real Time PCR},
  isbn={978-3-00-030214-5},
  publisher={Fördergemeinschaft Ökologischer Obstbau},
  address={Weinsberg},
  booktitle={Eco-Fruit : proceedings to the conference from February 22 nd to February 24 th, 2010 at the University of Hohenheim, Germany ; 14 th International Conference on Organic Fruit-Growing},
  pages={110--117},
  editor={Fördergemeinschaft Ökologischer Obstbau e. V.},
  author={Voegele, Ralf T. and Kunz, Stefan and Olbrecht, L. and Hinze, Malin and Weißhaupt, S. and Schmid, A. and Ernst, M. and Joos, M. and Matschinsky, M. and Mendgen, Kurt}
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