Publikation: Ca2+ oscillations mediated by exogenous GTP in Paramecium cells : assessment of possible Ca2+ sources
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We applied exogenous guanosine trisphosphate, GTP, to Paramecium tetraurelia cells injected with Fura Red for analysing changes of free intracellular Ca2+ concentrations, [Ca2+]i, during periodic back-/forward swimming thus induced. Strain ginA (non-responsive to GTP) shows no Ca2+ signal upon GTP application. In strain nd6 (normal Ca2+ signalling) an oscillating [Ca2+]i response with a prominent first peak occurs upon GTP stimulation, but none after mock-stimulation or after 15 min adaptation to GTP. While this is in agreement with previous electrophysiological analyses, we now try to identify more clearly the source(s) of Ca2+. Stimulation of nd6 cells, after depletion of Ca2+ from their cortical stores (alveolar sacs), shows the same Ca2+ oscillation pattern but with reduced amplitudes, and a normal behavioural response is observed. Stimulation with GTP, supplemented with the Ca2+ chelator BAPTA, results in loss of the first prominent Ca2+ peak, in reduction of the following Ca2+ amplitudes, and in the absence of any behavioural response. Both these observations strongly suggest that for the initiation of GTP-mediated back-/forward swimming Ca2+ from the extracellular medium is needed. For the maintenance of the Ca2+ oscillations a considerable fraction must come from internal stores, probably other than alveolar sacs, rather likely from the endoplasmic reticulum.
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SEHRING, Ivonne Margarete, Helmut PLATTNER, 2004. Ca2+ oscillations mediated by exogenous GTP in Paramecium cells : assessment of possible Ca2+ sources. In: Cell Calcium. 2004, 36(5), pp. 409-420. ISSN 0143-4160. eISSN 1532-1991BibTex
@article{Sehring2004oscil-6607, year={2004}, title={Ca2+ oscillations mediated by exogenous GTP in Paramecium cells : assessment of possible Ca2+ sources}, number={5}, volume={36}, issn={0143-4160}, journal={Cell Calcium}, pages={409--420}, author={Sehring, Ivonne Margarete and Plattner, Helmut} }
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