Syntrophe Oxidation der Fettsäure Acetat und des biogenen Amins Cadaverin (1,5-Diaminopentan) durch definierte methanogene Kokulturen

Abstract

In this work the anaerobic syntrohic oxidation of the fatty acid acetate and the biogenic amine cadaverine (1,5-diaminopentane) by two different defined cocultures was investigated. The manner of the anaerobic oxidation of the fatty acid acetate by the thermophilic coculture Synac should be clarified. Therefore the acetate fermenting bacterium Thermacetogenium phaeum was tested for the presence of the key enzymes of the Wood-Ljungdahl pathway in pure culture and comparatively in syntrophic grown cells. Distinctions of the enzymatic expression under both growing circumstances were investigated and depicted biochemically and proteomically. Proteins were sorted into cytoplasmic and membrane associated, or membrane integral proteins, respectively according to their distribution in the fractions. Based on this specific composition, conclusions about the possible energy gaining steps of the acetate degradation were drawn. A possible sodium dependance of the acetogenic bacterium T. phaeum should be investigated. The mesophilic coculture LC 13M, isolated from the enrichment culture La Cad, should be characterized physiologically and phylogenetically. The pathway of the cadaverine degradation should be mapped applying biochemical methods. The thereby forming products should be determined and quantified.