Publikation: Structural model of MalK, the ABC subunit of the maltose transporter of Escherichia coli : Implications for mal gene regulation, inducer exclusion and subunit assembly
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We are presenting a three-dimensional model of MalK, the ABC subunit of the maltose transporter from Escherichia coli and Salmonella typhimurium. It is based on the recently published crystal structure of the closely related Thermococcus litoralis MalK. The model was used to identify the position of mutations affecting the different functions of the ABC subunit. Six malK point mutations were isolated specifically affecting the interaction with MalT, the transcriptional regulator of the maltose system. They were mapped on the structural model and define a MalT interaction site that is located on an exposed surface of the C-terminal regulatory domain. Published point mutations that confer an inducer exclusion insensitive phenotype form a patch adjacent to and oriented perpendicularly to the MalT interaction site. Three sequence motifs were identified and visualized that are highly conserved among ABC subunits with extended C termini. They form a subdomain between the regulatory and ATPase domain and might play an important role in signal transduction events between these two domains. Mutations in this domain remain fully active in MalT regulation but cause transport defects. In addition, amino acids that have previously been shown to be involved in the interaction with the transmembranous subunits MalF and MalG and that fall into the highly conserved N-terminal ATPase domain were visualized. The validity of the modeled MalK structure was verified by structure-directed mutagenesis of amino acids located within the proposed MalK-MalT interaction site.
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BÖHM, Alex, Joachim DIEZ, Kay DIEDERICHS, Wolfram WELTE, Winfried BOOS, 2002. Structural model of MalK, the ABC subunit of the maltose transporter of Escherichia coli : Implications for mal gene regulation, inducer exclusion and subunit assembly. In: Journal of Biological Chemistry. 2002, 277(5), pp. 3708-3717. ISSN 0021-9258. eISSN 1083-351X. Available under: doi: 10.1074/jbc.M107905200BibTex
@article{Bohm2002Struc-8677, year={2002}, doi={10.1074/jbc.M107905200}, title={Structural model of MalK, the ABC subunit of the maltose transporter of Escherichia coli : Implications for mal gene regulation, inducer exclusion and subunit assembly}, number={5}, volume={277}, issn={0021-9258}, journal={Journal of Biological Chemistry}, pages={3708--3717}, author={Böhm, Alex and Diez, Joachim and Diederichs, Kay and Welte, Wolfram and Boos, Winfried} }
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