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Implications of active site constraints on varied DNA polymerase selectivity

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2002

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Strerath, Michael
Cramer, Janina
Restle, Tobias

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Journal of the American Chemical Society. 2002, 124(38), pp. 11230-11231. ISSN 0002-7863. Available under: doi: 10.1021/ja027060k

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DNA polymerase selectivity often varies significantly depending on the DNA polymerase. The origin of this varying error propensity is elusive. It is assumed that DNA polymerases form nucleotide binding pockets that differ in properties such as shape and tightness. We tested this prediction and studied HIV-1 RT by employment of size-augmented nucleotides and site-directed mutagenesis of the enzyme. New valuable insights into the mechanism of DNA polymerase fidelity were obtained. The presented study provides experimental evidence that variations of steric constraints within the nucleotide binding pocket of at least two DNA polymerases cause variations in nucleotide incorporation selectivity. Thus, our results support the concept of active site tightness as a causative in differential fidelity among DNA polymerases.

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540 Chemie

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ISO 690STRERATH, Michael, Janina CRAMER, Tobias RESTLE, Andreas MARX, 2002. Implications of active site constraints on varied DNA polymerase selectivity. In: Journal of the American Chemical Society. 2002, 124(38), pp. 11230-11231. ISSN 0002-7863. Available under: doi: 10.1021/ja027060k
BibTex
@article{Strerath2002Impli-16726,
  year={2002},
  doi={10.1021/ja027060k},
  title={Implications of active site constraints on varied DNA polymerase selectivity},
  number={38},
  volume={124},
  issn={0002-7863},
  journal={Journal of the American Chemical Society},
  pages={11230--11231},
  author={Strerath, Michael and Cramer, Janina and Restle, Tobias and Marx, Andreas}
}
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