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Synthesis and characterization of a nonapeptides with specific properties for Cu binding

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2007

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Drochioiu, Gabi
Murariu, Manuela

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http://nbn-resolving.de/urn:nbn:de:bsz:352-196718
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Chemie: Publikationen
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Published

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Revista de chimie : (The Chemistry Magazine). 2007, 58(3), pp. 311-315

Zusammenfassung

A nonapeptide with the following sequence of amino acid residues CHQYHHNRE has been synthesized and characterized. The peptide is useful to investigate the binding of copper ions to histidine-containing peptides and proteins. It was synthesised manually by Solid Phase Peptide Synthesis (SPPS) using Fmoc protection strategy. The peptide was subjected to final purification by Reversed-Phase High Performance Liquid Chromatography (RP-HPLC) and characterised by matrix assisted laser desorption ionization with time of flight mass spectrometry (MALDI-TOF MS). The GPMAW 6.11 (General Protein/Mass Analysis for Window) program was used for theoretical characterization of the peptide.

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Fachgebiet (DDC)
540 Chemie

Schlagwörter

peptide synthesis, Fmoc strategy, mass spectrometry, copper binding

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ISO 690DROCHIOIU, Gabi, Manuela MURARIU, Brînduşa-Alina PETRE, Marilena MANEA, Michael PRZYBYLSKI, 2007. Synthesis and characterization of a nonapeptides with specific properties for Cu binding. In: Revista de chimie : (The Chemistry Magazine). 2007, 58(3), pp. 311-315
BibTex
@article{Drochioiu2007Synth-19671,
  year={2007},
  title={Synthesis and characterization of a nonapeptides with specific properties for Cu binding},
  number={3},
  volume={58},
  journal={Revista de chimie : (The Chemistry Magazine)},
  pages={311--315},
  author={Drochioiu, Gabi and Murariu, Manuela and Petre, Brînduşa-Alina and Manea, Marilena and Przybylski, Michael}
}
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