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The oligomeric antenna of the diatom P. tricornutum—localisation of diadinoxanthin cycle pigments

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2008

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Volke, Daniela
Szábo, Milan
Hoffmann, Ralf
Garab, Gyözö
Wilhelm, Christian
Goss, Reimund

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ALLEN, John F., ed. and others. Photosynthesis: energy from the sun : 14th International Congress on Photosynthesis, Glasgow 2007 ; vol. 1. Dordrecht: Springer, 2008, pp. 283-286. ISBN 978-1-4020-6707-5. Available under: doi: 10.1007/978-1-4020-6709-9_63

Zusammenfassung

In the present work a thorough examination of the oligomeric antenna of P. tricornutum was performed. Solubilisation of thylakoids with n-dodecyl β-d-maltoside in combination with gelfiltration and sucrose density gradient centrifugation revealed the existence of two oligomeric antenna states, the FCPo and FCP complex. Calculation of the molecular masses showed that these complexes are organized as hexamers and trimers, respectively. By MS/MS analysis a specific assignment of the FCPs to their respective genes was achieved. The peripheral antenna contained the majority of the xanthophyll cycle pigments diadinoxanthin and diatoxanthin, while an additional small pool was tightly bound to the photosystem core complexes, indicating the existence of two distinct xanthophyll cycle pigment binding proteins.

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570 Biowissenschaften, Biologie

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14th International Congress on Photosynthesis, 22. Juli 2007 - 27. Juli 2007, Glasgow
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ISO 690LEPETIT, Bernard, Daniela VOLKE, Milan SZÁBO, Ralf HOFFMANN, Gyözö GARAB, Christian WILHELM, Reimund GOSS, 2008. The oligomeric antenna of the diatom P. tricornutum—localisation of diadinoxanthin cycle pigments. 14th International Congress on Photosynthesis. Glasgow, 22. Juli 2007 - 27. Juli 2007. In: ALLEN, John F., ed. and others. Photosynthesis: energy from the sun : 14th International Congress on Photosynthesis, Glasgow 2007 ; vol. 1. Dordrecht: Springer, 2008, pp. 283-286. ISBN 978-1-4020-6707-5. Available under: doi: 10.1007/978-1-4020-6709-9_63
BibTex
@inproceedings{Lepetit2008oligo-31037,
  year={2008},
  doi={10.1007/978-1-4020-6709-9_63},
  title={The oligomeric antenna of the diatom P. tricornutum—localisation of diadinoxanthin cycle pigments},
  isbn={978-1-4020-6707-5},
  publisher={Springer},
  address={Dordrecht},
  booktitle={Photosynthesis: energy from the sun : 14th International Congress on Photosynthesis, Glasgow 2007 ; vol. 1},
  pages={283--286},
  editor={Allen, John F.},
  author={Lepetit, Bernard and Volke, Daniela and Szábo, Milan and Hoffmann, Ralf and Garab, Gyözö and Wilhelm, Christian and Goss, Reimund}
}
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