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The Cre/Lox System to Assess the Development of the Mouse Brain

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2014

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Rijli, Filippo M.

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https://doi.org/10.1007/978-1-62703-655-9_20
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SPRECHER, Simon G, ed.. Brain Development : Methods and Protocols. Totowa, NJ: Humana Press, 2014, pp. 295-313. Methods in Molecular Biology. 1082. ISBN 978-1-62703-654-2. Available under: doi: 10.1007/978-1-62703-655-9_20

Zusammenfassung

Cre-mediated recombination has become a powerful tool to confine gene deletions (conditional knockouts) or overexpression of genes (conditional knockin/overexpression). By spatiotemporal restriction of genetic manipulations, major problems of classical knockouts such as embryonic lethality can be circumvented. Furthermore Cre-mediated recombination has broad applicability in the analysis of the cellular behavior of subpopulations and cell types as well as for genetic fate mapping. This chapter will give an overview about applications for the Cre/LoxP system and their execution.

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570 Biowissenschaften, Biologie

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ISO 690KRATOCHWIL, Claudius F., Filippo M. RIJLI, 2014. The Cre/Lox System to Assess the Development of the Mouse Brain. In: SPRECHER, Simon G, ed.. Brain Development : Methods and Protocols. Totowa, NJ: Humana Press, 2014, pp. 295-313. Methods in Molecular Biology. 1082. ISBN 978-1-62703-654-2. Available under: doi: 10.1007/978-1-62703-655-9_20
BibTex
@incollection{Kratochwil2014CreLo-33372,
  year={2014},
  doi={10.1007/978-1-62703-655-9_20},
  title={The Cre/Lox System to Assess the Development of the Mouse Brain},
  number={1082},
  isbn={978-1-62703-654-2},
  publisher={Humana Press},
  address={Totowa, NJ},
  series={Methods in Molecular Biology},
  booktitle={Brain Development : Methods and Protocols},
  pages={295--313},
  editor={Sprecher, Simon G},
  author={Kratochwil, Claudius F. and Rijli, Filippo M.}
}
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