Post-cryopreservation viability of the benthic freshwater diatom Planothidium frequentissimum depends on light levels

Buhmann, Matthias T.; Day, John G.; Kroth, Peter G.

Post-cryopreservation viability of the benthic freshwater diatom Planothidium frequentissimum depends on light levels

Dateien

Buhmann_263384.pdfGröße: 587.04 KBDownloads: 574

Datum

2013

Autor:innen

Buhmann, Matthias T.

Day, John G.

Kroth, Peter G.

Publikationstyp

Zeitschriftenartikel

unikn.publication.listelement.citation.prefix.version.undefined

Cryobiology. 2013, 67(1), pp. 23-29. ISSN 0011-2240. eISSN 1090-2392. Available under: doi: 10.1016/j.cryobiol.2013.04.005

Zusammenfassung

Over recent years, several planktonic and benthic freshwater diatom taxa have been established as laboratory model strains. In common with most freshwater diatoms the pennate diatom Planothidium frequentissimum suffers irreversible cell shrinkage on prolonged maintenance by serial transfers, without induction of the sexual cycle. Therefore, alternative strategies are required for the long-term maintenance of this strain. Conventional colligative cryopreservation approaches have previously proven unsuccessful with no regrowth. However, in this study using 5% dimethyl sulfoxide (Me₂SO), controlled cooling at 1 °C min⁻¹, automated ice seeding and cooling to −40 °C with a final plunge into liquid nitrogen, viability levels were enhanced from 0.3 ± 0.4% to 80 ± 3%, by incorporating a 48 h dark-recovery phase after rewarming. Omission, or reduction, of this recovery step resulted in obvious cell damage with photo-bleaching of pigments, indicative of oxidative-stress induced cell damage, with subsequent deterioration of cellular architecture.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Zitieren

ISO 690

BUHMANN, Matthias T., John G. DAY, Peter G. KROTH, 2013. Post-cryopreservation viability of the benthic freshwater diatom Planothidium frequentissimum depends on light levels. In: Cryobiology. 2013, 67(1), pp. 23-29. ISSN 0011-2240. eISSN 1090-2392. Available under: doi: 10.1016/j.cryobiol.2013.04.005

BibTex

@article{Buhmann2013-08Postc-26338,
  year={2013},
  doi={10.1016/j.cryobiol.2013.04.005},
  title={Post-cryopreservation viability of the benthic freshwater diatom Planothidium frequentissimum depends on light levels},
  number={1},
  volume={67},
  issn={0011-2240},
  journal={Cryobiology},
  pages={23--29},
  author={Buhmann, Matthias T. and Day, John G. and Kroth, Peter G.}
}

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