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Preparation and Application of Enzyme-Nucleotide Conjugates

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2018

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https://doi.org/10.1002/cpch.36
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Current protocols in chemical biology. 2018, 10(1), pp. 49-71. eISSN 2160-4762. Available under: doi: 10.1002/cpch.36

Zusammenfassung

In this unit the preparation and application of enzyme-nucleotide conjugates is depicted. First, a modified nucleoside triphosphate is synthesized bearing a long and flexible linker equipped with a thiol group. The nucleotide is then reacted with maleimide-activated horseradish peroxidase to yield an enzyme-nucleotide conjugate, which due to the long linker, can be used as a substrate by DNA polymerases in primer extension reactions. Finally, an assay based on these findings is described that provides a fast and easy nucleic acid detection and genotyping platform.

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Fachgebiet (DDC)
540 Chemie

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DNA polymerase; Nucleotides; naked eyes detection; nucleic acid diagnostics; protein conjugates

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ISO 690WELTER, Moritz, Andreas MARX, 2018. Preparation and Application of Enzyme-Nucleotide Conjugates. In: Current protocols in chemical biology. 2018, 10(1), pp. 49-71. eISSN 2160-4762. Available under: doi: 10.1002/cpch.36
BibTex
@article{Welter2018Prepa-44590,
  year={2018},
  doi={10.1002/cpch.36},
  title={Preparation and Application of Enzyme-Nucleotide Conjugates},
  number={1},
  volume={10},
  journal={Current protocols in chemical biology},
  pages={49--71},
  author={Welter, Moritz and Marx, Andreas}
}
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