Publikation:

Dissociated neurons of the pupal honeybee brain in cell culture

Lade...
Vorschaubild

Dateien

jofneurokreisslbicker.pdf
jofneurokreisslbicker.pdfGröße: 4.66 MBDownloads: 667

Datum

1992

Autor:innen

Bicker, Gerd

Herausgeber:innen

Kontakt

ISSN der Zeitschrift

Electronic ISSN

ISBN

Bibliografische Daten

Verlag

Schriftenreihe

Auflagebezeichnung

DOI (zitierfähiger Link)
ArXiv-ID

Internationale Patentnummer

Angaben zur Forschungsförderung

Projekt

Open Access-Veröffentlichung
Open Access Green
Core Facility der Universität Konstanz

Gesperrt bis

Titel in einer weiteren Sprache

Publikationstyp
Zeitschriftenartikel
Publikationsstatus
Published

Erschienen in

Journal of Neurocytology. 1992, 21(8), pp. 545-556. ISSN 0300-4864. Available under: doi: 10.1007/BF01187116

Zusammenfassung

Primary cell cultures were prepared from specific regions of the pupal honeybee brain which are involved in proboscis extension learning. Defined areas could be dissociated purely by mechanical treatment. We show that cultured neurons regenerate new neurites and remain viable for up to three weeks in a serum-free, chemically-defined medium. Several labelling techniques were employed to identify subpopulations of cultured neurons. For example, acetylcholinesterase staining; fluorescent beads to distinguish identified cell populations of co-cultured brain areas; various markers for surface antigens such as a monoclonal antibody to olfactory projection neurons of the antennoglomerular tracts and monopolar cells of the optic lobes, as well as anti-HRP immunoreactivity and agr-bungarotoxin binding; and various antisera for detecting transmitter phenotype. The appearance of transmitter-immunoreactive cells agreed closely with that expected from their known distributionin situ. Our results suggest that cultured cells retain surface properties and transmitter phenotype of theirin vivo counterparts, despite differences in basic morphology. Thus our culture system provides the important initial step for futurein vitro investigations of the cellular and electrophysiological properties of neurons mediating proboscis extension learning.

Zusammenfassung in einer weiteren Sprache

Fachgebiet (DDC)
570 Biowissenschaften, Biologie

Schlagwörter

Konferenz

Rezension
undefined / . - undefined, undefined

Forschungsvorhaben

Organisationseinheiten

Zeitschriftenheft

Zugehörige Datensätze in KOPS

Zitieren

ISO 690KREISSL, Sabine, Gerd BICKER, 1992. Dissociated neurons of the pupal honeybee brain in cell culture. In: Journal of Neurocytology. 1992, 21(8), pp. 545-556. ISSN 0300-4864. Available under: doi: 10.1007/BF01187116
BibTex
@article{Kreil1992Disso-16329,
  year={1992},
  doi={10.1007/BF01187116},
  title={Dissociated neurons of the pupal honeybee brain in cell culture},
  number={8},
  volume={21},
  issn={0300-4864},
  journal={Journal of Neurocytology},
  pages={545--556},
  author={Kreißl, Sabine and Bicker, Gerd}
}
RDF
<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/16329">
    <dcterms:issued>1992</dcterms:issued>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/16329/1/jofneurokreisslbicker.pdf"/>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-10-13T12:28:20Z</dcterms:available>
    <dc:rights>terms-of-use</dc:rights>
    <dc:language>eng</dc:language>
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/16329/1/jofneurokreisslbicker.pdf"/>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dcterms:title>Dissociated neurons of the pupal honeybee brain in cell culture</dcterms:title>
    <dc:creator>Kreißl, Sabine</dc:creator>
    <dc:creator>Bicker, Gerd</dc:creator>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dcterms:abstract xml:lang="eng">Primary cell cultures were prepared from specific regions of the pupal honeybee brain which are involved in proboscis extension learning. Defined areas could be dissociated purely by mechanical treatment. We show that cultured neurons regenerate new neurites and remain viable for up to three weeks in a serum-free, chemically-defined medium. Several labelling techniques were employed to identify subpopulations of cultured neurons. For example, acetylcholinesterase staining; fluorescent beads to distinguish identified cell populations of co-cultured brain areas; various markers for surface antigens such as a monoclonal antibody to olfactory projection neurons of the antennoglomerular tracts and monopolar cells of the optic lobes, as well as anti-HRP immunoreactivity and agr-bungarotoxin binding; and various antisera for detecting transmitter phenotype. The appearance of transmitter-immunoreactive cells agreed closely with that expected from their known distributionin situ. Our results suggest that cultured cells retain surface properties and transmitter phenotype of theirin vivo counterparts, despite differences in basic morphology. Thus our culture system provides the important initial step for futurein vitro investigations of the cellular and electrophysiological properties of neurons mediating proboscis extension learning.</dcterms:abstract>
    <dc:contributor>Kreißl, Sabine</dc:contributor>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-10-13T12:28:20Z</dc:date>
    <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/16329"/>
    <dc:contributor>Bicker, Gerd</dc:contributor>
    <dcterms:bibliographicCitation>First publ. in: Journal of Neurocytology ; 21 (1992), 8. - pp. 545-556</dcterms:bibliographicCitation>
  </rdf:Description>
</rdf:RDF>

Interner Vermerk

xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter

Kontakt
URL der Originalveröffentl.

Prüfdatum der URL

Prüfungsdatum der Dissertation

Finanzierungsart

Kommentar zur Publikation

Allianzlizenz
Corresponding Authors der Uni Konstanz vorhanden
Internationale Co-Autor:innen
Universitätsbibliographie
Nein
Begutachtet
Diese Publikation teilen