A Model for Carbohydrate Metabolism in the Diatom Phaeodactylum tricornutum Deduced from Comparative Whole Genome Analysis

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2008
Autor:innen
Chiovitti, Anthony
Martin-Jezequel, Veronique
Mock, Thomas
Schnitzler Parker, Micaela
Stanley, Michele S.
Kaplan, Aaron
Caron, Lise
Weber, Till
Herausgeber:innen
Kroymann, Juergen
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Zusammenfassung

Background:
Diatoms are unicellular algae responsible for approximately 20% of global carbon fixation. Their evolution by secondary endocytobiosis resulted in a complex cellular structure and metabolism compared to algae with primary plastids.
Methodology/Principal Findings:
The whole genome sequence of the diatom Phaeodactylum tricornutum has recently been completed. We identified and annotated genes for enzymes involved in carbohydrate pathways based on extensive EST support and comparison to the whole genome sequence of a second diatom, Thalassiosira pseudonana. Protein localization to mitochondria was predicted based on identified similarities to mitochondrial localization motifs in other eukaryotes, whereas protein localization to plastids was based on the presence of signal peptide motifs in combination with plastid localization motifs previously shown to be required in diatoms. We identified genes potentially involved in a C4-like photosynthesis in P. tricornutum and, on the basis of sequence-based putative localization of relevant proteins, discuss possible differences in carbon concentrating mechanisms and CO2 fixation between the two diatoms. We also identified genes encoding enzymes involved in photorespiration with one interesting exception: glycerate kinase was not found in either P. tricornutum or T. pseudonana. Various Calvin cycle enzymes were found in up to five different isoforms, distributed between plastids, mitochondria and the cytosol. Diatoms store energy either as lipids or as chrysolaminaran (a β-1,3-glucan) outside of the plastids. We identified various β-glucanases and large membrane-bound glucan synthases. Interestingly most of the glucanases appear to contain C-terminal anchor domains that may attach the enzymes to membranes.
Conclusions/Significance:
Here we present a detailed synthesis of carbohydrate metabolism in diatoms based on the genome sequences of Thalassiosira pseudonana and Phaeodactylum tricornutum. This model provides novel insights into acquisition of dissolved inorganic carbon and primary metabolic pathways of carbon in two different diatoms, which is of significance for an improved understanding of global carbon cycles.

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570 Biowissenschaften, Biologie
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ISO 690KROTH, Peter G., Anthony CHIOVITTI, Ansgar GRUBER, Veronique MARTIN-JEZEQUEL, Thomas MOCK, Micaela SCHNITZLER PARKER, Michele S. STANLEY, Aaron KAPLAN, Lise CARON, Till WEBER, Uma MAHESWARI, Elisabeth Virginia ARMBRUST, Chris BOWLER, KROYMANN, Juergen, ed., 2008. A Model for Carbohydrate Metabolism in the Diatom Phaeodactylum tricornutum Deduced from Comparative Whole Genome Analysis. In: PLoS ONE. 2008, 3(1), pp. e1426. eISSN 1932-6203. Available under: doi: 10.1371/journal.pone.0001426
BibTex
@article{Kroth2008Model-7941,
  year={2008},
  doi={10.1371/journal.pone.0001426},
  title={A Model for Carbohydrate Metabolism in the Diatom Phaeodactylum tricornutum Deduced from Comparative Whole Genome Analysis},
  number={1},
  volume={3},
  journal={PLoS ONE},
  author={Kroth, Peter G. and Chiovitti, Anthony and Gruber, Ansgar and Martin-Jezequel, Veronique and Mock, Thomas and Schnitzler Parker, Micaela and Stanley, Michele S. and Kaplan, Aaron and Caron, Lise and Weber, Till and Maheswari, Uma and Armbrust, Elisabeth Virginia and Bowler, Chris},
  note={Article Number: e1426}
}
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