Publikation:

Exploring Obscurin and SPEG Kinase Biology

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2021

Autor:innen

Rani, Alankrita
Kraft, Jamie
Zenker, Sanja
Börgeson, Emma
Lange, Stephan

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Published

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Journal of Clinical Medicine. MDPI. 2021, 10(5), 984. eISSN 2077-0383. Available under: doi: 10.3390/jcm10050984

Zusammenfassung

Three members of the obscurin protein family that contain tandem kinase domains with important signaling functions for cardiac and striated muscles are the giant protein obscurin, its obscurin-associated kinase splice isoform, and the striated muscle enriched protein kinase (SPEG). While there is increasing evidence for the specific roles that each individual kinase domain plays in cross-striated muscles, their biology and regulation remains enigmatic. Our present study focuses on kinase domain 1 and the adjacent low sequence complexity inter-kinase domain linker in obscurin and SPEG. Using Phos-tag gels, we show that the linker in obscurin contains several phosphorylation sites, while the same region in SPEG remained unphosphorylated. Our homology modeling, mutational analysis and molecular docking demonstrate that kinase 1 in obscurin harbors all key amino acids important for its catalytic function and that actions of this domain result in autophosphorylation of the protein. Our bioinformatics analyses also assign a list of putative substrates for kinase domain 1 in obscurin and SPEG, based on the known and our newly proposed phosphorylation sites in muscle proteins, including obscurin itself.

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570 Biowissenschaften, Biologie

Schlagwörter

obscurin; striated muscle enriched protein kinase; SPEG; kinase

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ISO 690FLEMING, Jennifer R., Alankrita RANI, Jamie KRAFT, Sanja ZENKER, Emma BÖRGESON, Stephan LANGE, 2021. Exploring Obscurin and SPEG Kinase Biology. In: Journal of Clinical Medicine. MDPI. 2021, 10(5), 984. eISSN 2077-0383. Available under: doi: 10.3390/jcm10050984
BibTex
@article{Fleming2021-03-02Explo-53254,
  year={2021},
  doi={10.3390/jcm10050984},
  title={Exploring Obscurin and SPEG Kinase Biology},
  number={5},
  volume={10},
  journal={Journal of Clinical Medicine},
  author={Fleming, Jennifer R. and Rani, Alankrita and Kraft, Jamie and Zenker, Sanja and Börgeson, Emma and Lange, Stephan},
  note={Article Number: 984}
}
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