Publikation:

Preferential cultivation of type II methanotrophic bacteria from littoral sediments (Lake Constance)

Lade...
Vorschaubild

Dateien

2004_Bussmann_179_189.pdf
2004_Bussmann_179_189.pdfGröße: 320.98 KBDownloads: 348

Datum

2004

Herausgeber:innen

Kontakt

ISSN der Zeitschrift

Electronic ISSN

ISBN

Bibliografische Daten

Verlag

Schriftenreihe

Auflagebezeichnung

ArXiv-ID

Internationale Patentnummer

Angaben zur Forschungsförderung

Projekt

Open Access-Veröffentlichung
Open Access Green
Core Facility der Universität Konstanz

Gesperrt bis

Titel in einer weiteren Sprache

Publikationstyp
Zeitschriftenartikel
Publikationsstatus
Published

Erschienen in

FEMS Microbiology Ecology. 2004, 47(2), pp. 179-189. eISSN 1574-6941. Available under: doi: 10.1016/S0168-6496(03)00260-5

Zusammenfassung

Most widely used medium for cultivation of methanotrophic bacteria from various environments is that proposed in 1970 by Whittenbury. In order to adapt and optimize medium for culturing of methanotrophs from freshwater sediment, media with varying concentrations of substrates, phosphate, nitrate, and other mineral salts were used to enumerate methanotrophs by the most probable number method. High concentrations (s1 mM) of magnesium and sulfate, and high concentrations of nitrate (s500 WM) significantly reduced the number of cultured methanotrophs, whereas phosphate in the range of 15^1500 WM had no influence. Also oxygen and carbon dioxide influenced the culturing efficiency, with an optimal mixing ratio of 17% O2 and 3% CO2 ; the mixing ratio of methane (6^32%) had no effect. A clone library of pmoA genes amplified by PCR from DNA extracted from sediment revealed the presence of both type I and type II methanotrophs. Nonetheless, the cultivation of methanotrophs, also with the improved medium, clearly favored growth of type II methanotrophs of the Methylosinus/Methylocystis group. Although significantly more methanotrophs could be cultured with the modified medium, their diversity did not mirror the diversity of methanotrophs in the sediment sample detected by molecular biology method.

Zusammenfassung in einer weiteren Sprache

Fachgebiet (DDC)
570 Biowissenschaften, Biologie

Schlagwörter

Cultivation, methanotroph, medium composition, terminal restriction fragment length polymorphism, sediment

Konferenz

Rezension
undefined / . - undefined, undefined

Forschungsvorhaben

Organisationseinheiten

Zeitschriftenheft

Zugehörige Datensätze in KOPS

Zitieren

ISO 690BUSSMANN, Ingeborg, Michael PESTER, Andreas BRUNE, Bernhard SCHINK, 2004. Preferential cultivation of type II methanotrophic bacteria from littoral sediments (Lake Constance). In: FEMS Microbiology Ecology. 2004, 47(2), pp. 179-189. eISSN 1574-6941. Available under: doi: 10.1016/S0168-6496(03)00260-5
BibTex
@article{Bussmann2004Prefe-7634,
  year={2004},
  doi={10.1016/S0168-6496(03)00260-5},
  title={Preferential cultivation of type II methanotrophic bacteria from littoral sediments (Lake Constance)},
  number={2},
  volume={47},
  journal={FEMS Microbiology Ecology},
  pages={179--189},
  author={Bussmann, Ingeborg and Pester, Michael and Brune, Andreas and Schink, Bernhard}
}
RDF
<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/7634">
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:35:56Z</dc:date>
    <dc:creator>Brune, Andreas</dc:creator>
    <dc:format>application/pdf</dc:format>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/7634/1/2004_Bussmann_179_189.pdf"/>
    <dc:contributor>Brune, Andreas</dc:contributor>
    <dc:language>eng</dc:language>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dc:creator>Schink, Bernhard</dc:creator>
    <dcterms:bibliographicCitation>First publ. in: FEMS Microbiology Ecology 47 (2004), 2, pp. 179-189</dcterms:bibliographicCitation>
    <dc:contributor>Pester, Michael</dc:contributor>
    <dcterms:issued>2004</dcterms:issued>
    <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:35:56Z</dcterms:available>
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/7634/1/2004_Bussmann_179_189.pdf"/>
    <dc:creator>Bussmann, Ingeborg</dc:creator>
    <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/7634"/>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dcterms:abstract xml:lang="eng">Most widely used medium for cultivation of methanotrophic bacteria from various environments is that proposed in 1970 by Whittenbury. In order to adapt and optimize medium for culturing of methanotrophs from freshwater sediment, media with varying concentrations of substrates, phosphate, nitrate, and other mineral salts were used to enumerate methanotrophs by the most probable number method. High concentrations (s1 mM) of magnesium and sulfate, and high concentrations of nitrate (s500 WM) significantly reduced the number of cultured methanotrophs, whereas phosphate in the range of 15^1500 WM had no influence. Also oxygen and carbon dioxide influenced the culturing efficiency, with an optimal mixing ratio of 17% O2 and 3% CO2 ; the mixing ratio of methane (6^32%) had no effect. A clone library of pmoA genes amplified by PCR from DNA extracted from sediment revealed the presence of both type I and type II methanotrophs. Nonetheless, the cultivation of methanotrophs, also with the improved medium, clearly favored growth of type II methanotrophs of the Methylosinus/Methylocystis group. Although significantly more methanotrophs could be cultured with the modified medium, their diversity did not mirror the diversity of methanotrophs in the sediment sample detected by molecular biology method.</dcterms:abstract>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dc:rights>terms-of-use</dc:rights>
    <dc:creator>Pester, Michael</dc:creator>
    <dc:contributor>Schink, Bernhard</dc:contributor>
    <dc:contributor>Bussmann, Ingeborg</dc:contributor>
    <dcterms:title>Preferential cultivation of type II methanotrophic bacteria from littoral sediments (Lake Constance)</dcterms:title>
  </rdf:Description>
</rdf:RDF>

Interner Vermerk

xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter

Kontakt
URL der Originalveröffentl.

Prüfdatum der URL

Prüfungsdatum der Dissertation

Finanzierungsart

Kommentar zur Publikation

Allianzlizenz
Corresponding Authors der Uni Konstanz vorhanden
Internationale Co-Autor:innen
Universitätsbibliographie
Ja
Begutachtet
Diese Publikation teilen