Publikation:

Genome editing in diatoms : achievements and goals

Vorschaubild

Dateien

Kroth_2-1lxrzq3xck4wo8.pdf
Kroth_2-1lxrzq3xck4wo8.pdfGröße: 183.17 KBDownloads: 1034

Datum

2018

Autor:innen

Bones, Atle M.
Daboussi, Fayza
Ferrante, Maria I.
Jaubert, Marianne
Kolot, Misha
Nymark, Marianne
Falciatore, Angela
et al.

Herausgeber:innen

Kontakt

ISSN der Zeitschrift

Electronic ISSN

ISBN

Bibliografische Daten

Verlag

Schriftenreihe

Auflagebezeichnung

URI (zitierfähiger Link)
http://nbn-resolving.de/urn:nbn:de:bsz:352-2-1lxrzq3xck4wo8
DOI (zitierfähiger Link)
https://doi.org/10.1007/s00299-018-2334-1
ArXiv-ID

Internationale Patentnummer

Link zur Lizenz
Urheberrechtlich geschützt

Angaben zur Forschungsförderung

Projekt

Open Access-Veröffentlichung
Open Access Green

Sammlungen

Biologie: Publikationen
Core Facility der Universität Konstanz

Gesperrt bis

Titel in einer weiteren Sprache

Publikationstyp
Zeitschriftenartikel
Publikationsstatus
Published

Erschienen in

Plant Cell Reports. 2018, 37(10), pp. 1401-1408. ISSN 0721-7714. eISSN 1432-203X. Available under: doi: 10.1007/s00299-018-2334-1

Zusammenfassung

Diatoms are major components of phytoplankton and play a key role in the ecology of aquatic ecosystems. These algae are of great scientific importance for a wide variety of research areas, ranging from marine ecology and oceanography to biotechnology. During the last 20 years, the availability of genomic information on selected diatom species and a substantial progress in genetic manipulation, strongly contributed to establishing diatoms as molecular model organisms for marine biology research. Recently, tailored TALEN endonucleases and the CRISPR/Cas9 system were utilized in diatoms, allowing targeted genetic modifications and the generation of knockout strains. These approaches are extremely valuable for diatom research because breeding, forward genetic screens by random insertion, and chemical mutagenesis are not applicable to the available model species Phaeodactylum tricornutum and Thalassiosira pseudonana, which do not cross sexually in the lab. Here, we provide an overview of the genetic toolbox that is currently available for performing stable genetic modifications in diatoms. We also discuss novel challenges that need to be addressed to fully exploit the potential of these technologies for the characterization of diatom biology and for metabolic engineering.

Zusammenfassung in einer weiteren Sprache

Fachgebiet (DDC)
570 Biowissenschaften, Biologie

Schlagwörter

Konferenz

Rezension
undefined / . - undefined, undefined

Forschungsvorhaben

Organisationseinheiten

Zeitschriftenheft

Verknüpfte Datensätze

Zitieren

ISO 690KROTH, Peter G., Atle M. BONES, Fayza DABOUSSI, Maria I. FERRANTE, Marianne JAUBERT, Misha KOLOT, Marianne NYMARK, Carolina RÍO BÁRTULOS, Manuel SERIF, Angela FALCIATORE, 2018. Genome editing in diatoms : achievements and goals. In: Plant Cell Reports. 2018, 37(10), pp. 1401-1408. ISSN 0721-7714. eISSN 1432-203X. Available under: doi: 10.1007/s00299-018-2334-1
BibTex
@article{Kroth2018-10Genom-44586,
  year={2018},
  doi={10.1007/s00299-018-2334-1},
  title={Genome editing in diatoms : achievements and goals},
  number={10},
  volume={37},
  issn={0721-7714},
  journal={Plant Cell Reports},
  pages={1401--1408},
  author={Kroth, Peter G. and Bones, Atle M. and Daboussi, Fayza and Ferrante, Maria I. and Jaubert, Marianne and Kolot, Misha and Nymark, Marianne and Río Bártulos, Carolina and Serif, Manuel and Falciatore, Angela}
}
RDF
<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/44586">
    <dc:creator>Ferrante, Maria I.</dc:creator>
    <dc:rights>terms-of-use</dc:rights>
    <dc:contributor>Falciatore, Angela</dc:contributor>
    <dc:contributor>Nymark, Marianne</dc:contributor>
    <dc:contributor>Río Bártulos, Carolina</dc:contributor>
    <dcterms:issued>2018-10</dcterms:issued>
    <dc:creator>Jaubert, Marianne</dc:creator>
    <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/44586"/>
    <dc:contributor>Bones, Atle M.</dc:contributor>
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/44586/1/Kroth_2-1lxrzq3xck4wo8.pdf"/>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dc:contributor>Daboussi, Fayza</dc:contributor>
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/44586/1/Kroth_2-1lxrzq3xck4wo8.pdf"/>
    <dc:contributor>Jaubert, Marianne</dc:contributor>
    <dc:creator>Daboussi, Fayza</dc:creator>
    <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/>
    <dc:language>eng</dc:language>
    <dc:creator>Río Bártulos, Carolina</dc:creator>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:creator>Falciatore, Angela</dc:creator>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:creator>Kolot, Misha</dc:creator>
    <dc:contributor>Ferrante, Maria I.</dc:contributor>
    <dc:creator>Bones, Atle M.</dc:creator>
    <dc:creator>Nymark, Marianne</dc:creator>
    <dcterms:abstract xml:lang="eng">Diatoms are major components of phytoplankton and play a key role in the ecology of aquatic ecosystems. These algae are of great scientific importance for a wide variety of research areas, ranging from marine ecology and oceanography to biotechnology. During the last 20 years, the availability of genomic information on selected diatom species and a substantial progress in genetic manipulation, strongly contributed to establishing diatoms as molecular model organisms for marine biology research. Recently, tailored TALEN endonucleases and the CRISPR/Cas9 system were utilized in diatoms, allowing targeted genetic modifications and the generation of knockout strains. These approaches are extremely valuable for diatom research because breeding, forward genetic screens by random insertion, and chemical mutagenesis are not applicable to the available model species Phaeodactylum tricornutum and Thalassiosira pseudonana, which do not cross sexually in the lab. Here, we provide an overview of the genetic toolbox that is currently available for performing stable genetic modifications in diatoms. We also discuss novel challenges that need to be addressed to fully exploit the potential of these technologies for the characterization of diatom biology and for metabolic engineering.</dcterms:abstract>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dc:contributor>Kolot, Misha</dc:contributor>
    <dc:contributor>Serif, Manuel</dc:contributor>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2019-01-16T09:16:55Z</dcterms:available>
    <dcterms:title>Genome editing in diatoms : achievements and goals</dcterms:title>
    <dc:contributor>Kroth, Peter G.</dc:contributor>
    <dc:creator>Kroth, Peter G.</dc:creator>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2019-01-16T09:16:55Z</dc:date>
    <dc:creator>Serif, Manuel</dc:creator>
  </rdf:Description>
</rdf:RDF>

Interner Vermerk

xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter

Kontakt
URL der Originalveröffentl.

Prüfdatum der URL

Prüfungsdatum der Dissertation

Finanzierungsart

Kommentar zur Publikation

Allianzlizenz
Corresponding Authors der Uni Konstanz vorhanden
Internationale Co-Autor:innen
Universitätsbibliographie
Ja
Begutachtet
Ja
Diese Publikation teilen