Genome editing in diatoms : achievements and goals

Kroth, Peter G.; Bones, Atle M.; Daboussi, Fayza; Ferrante, Maria I.; Jaubert, Marianne; Kolot, Misha; Nymark, Marianne; Río Bártulos, Carolina; Serif, Manuel; Falciatore, Angela

Genome editing in diatoms : achievements and goals

Dateien

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Datum

2018

Autor:innen

Kroth, Peter G.

Bones, Atle M.

Daboussi, Fayza

Ferrante, Maria I.

Jaubert, Marianne

Kolot, Misha

Nymark, Marianne

Río Bártulos, Carolina

Serif, Manuel

Falciatore, Angela

et al.

Open Access-Veröffentlichung

Open Access Green

Sammlungen

Biologie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

Plant Cell Reports. 2018, 37(10), pp. 1401-1408. ISSN 0721-7714. eISSN 1432-203X. Available under: doi: 10.1007/s00299-018-2334-1

Zusammenfassung

Diatoms are major components of phytoplankton and play a key role in the ecology of aquatic ecosystems. These algae are of great scientific importance for a wide variety of research areas, ranging from marine ecology and oceanography to biotechnology. During the last 20 years, the availability of genomic information on selected diatom species and a substantial progress in genetic manipulation, strongly contributed to establishing diatoms as molecular model organisms for marine biology research. Recently, tailored TALEN endonucleases and the CRISPR/Cas9 system were utilized in diatoms, allowing targeted genetic modifications and the generation of knockout strains. These approaches are extremely valuable for diatom research because breeding, forward genetic screens by random insertion, and chemical mutagenesis are not applicable to the available model species Phaeodactylum tricornutum and Thalassiosira pseudonana, which do not cross sexually in the lab. Here, we provide an overview of the genetic toolbox that is currently available for performing stable genetic modifications in diatoms. We also discuss novel challenges that need to be addressed to fully exploit the potential of these technologies for the characterization of diatom biology and for metabolic engineering.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Zitieren

ISO 690

KROTH, Peter G., Atle M. BONES, Fayza DABOUSSI, Maria I. FERRANTE, Marianne JAUBERT, Misha KOLOT, Marianne NYMARK, Carolina RÍO BÁRTULOS, Manuel SERIF, Angela FALCIATORE, 2018. Genome editing in diatoms : achievements and goals. In: Plant Cell Reports. 2018, 37(10), pp. 1401-1408. ISSN 0721-7714. eISSN 1432-203X. Available under: doi: 10.1007/s00299-018-2334-1

BibTex

@article{Kroth2018-10Genom-44586,
  year={2018},
  doi={10.1007/s00299-018-2334-1},
  title={Genome editing in diatoms : achievements and goals},
  number={10},
  volume={37},
  issn={0721-7714},
  journal={Plant Cell Reports},
  pages={1401--1408},
  author={Kroth, Peter G. and Bones, Atle M. and Daboussi, Fayza and Ferrante, Maria I. and Jaubert, Marianne and Kolot, Misha and Nymark, Marianne and Río Bártulos, Carolina and Serif, Manuel and Falciatore, Angela}
}

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Universitätsbibliographie

Ja

Begutachtet

Ja