Volutin granules of Eimeria parasites are acidic compartments and have physiological and structural characteristics similar to Acidocalcisomes

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2011
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Soares Medeiros, Lia Carolina
Gomes, Fabio
Maia Maciel, Luis Renato
Seabra, Sergio Henrique
Docampo, Roberto
Moreno, Silvia
Kawazoe, Urara
Barrabin, Hector
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Journal of Eukaryotic Microbiology. 2011, 58(5), pp. 416-423. ISSN 1066-5234. eISSN 1550-7408. Available under: doi: 10.1111/j.1550-7408.2011.00565.x
Zusammenfassung

The structural organization of parasites has been the subject of investigation by many groups and has lead to the identification of structures and metabolic pathways that may represent targets for anti-parasitic drugs. A specific group of organelles named acidocalcisomes has been identified in a number of organisms, including the apicomplexan parasites such as Toxoplasma and Plasmodium, where they have been shown to be involved in cation homeostasis, polyphosphate metabolism, and osmoregulation. Their structural counterparts in the apicomplexan parasite Eimeria have not been fully characterized. In this work, the ultrastructural and chemical properties of acidocalcisomes in Eimeria were characterized. Electron microscopy analysis of Eimeria parasites showed the dense organelles called volutin granules similar to acidocalcisomes. Immunolocalization of the vacuolar proton pyrophosphatase, considered as a marker for acidocalcisomes, showed labeling in vesicles of size and distribution similar to the dense organelles seen by electron microscopy. Spectrophotometric measurements of the kinetics of proton uptake showed a vacuolar proton pyrophosphatase activity. X-ray mapping revealed significant amounts of Na, Mg, P, K, Ca, and Zn in their matrix. The results suggest that volutin granules of Eimeria parasites are acidic, dense organelles, and possess structural and chemical properties analogous to those of other acidocalcisomes, suggesting a similar functional role in these parasites.

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Fachgebiet (DDC)
570 Biowissenschaften, Biologie
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Acidocalcisome, Eimeria acervulina, Eimeria tenella, elemental mapping, vacuolar proton pyrophosphatase, volutin granule
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ISO 690SOARES MEDEIROS, Lia Carolina, Fabio GOMES, Luis Renato MAIA MACIEL, Sergio Henrique SEABRA, Roberto DOCAMPO, Silvia MORENO, Helmut PLATTNER, Joachim HENTSCHEL, Urara KAWAZOE, Hector BARRABIN, Wanderley DE SOUZA, Renato Augusto DAMATTA, Kildare MIRANDA, 2011. Volutin granules of Eimeria parasites are acidic compartments and have physiological and structural characteristics similar to Acidocalcisomes. In: Journal of Eukaryotic Microbiology. 2011, 58(5), pp. 416-423. ISSN 1066-5234. eISSN 1550-7408. Available under: doi: 10.1111/j.1550-7408.2011.00565.x
BibTex
@article{SoaresMedeiros2011Volut-17830,
  year={2011},
  doi={10.1111/j.1550-7408.2011.00565.x},
  title={Volutin granules of Eimeria parasites are acidic compartments and have physiological and structural characteristics similar to Acidocalcisomes},
  number={5},
  volume={58},
  issn={1066-5234},
  journal={Journal of Eukaryotic Microbiology},
  pages={416--423},
  author={Soares Medeiros, Lia Carolina and Gomes, Fabio and Maia Maciel, Luis Renato and Seabra, Sergio Henrique and Docampo, Roberto and Moreno, Silvia and Plattner, Helmut and Hentschel, Joachim and Kawazoe, Urara and Barrabin, Hector and de Souza, Wanderley and Damatta, Renato Augusto and Miranda, Kildare}
}
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