Publikation:

Concanavalin A - induced T-cell - mediated hepatic injury in mice : The role of tumor necrosis factor

Lade...
Vorschaubild

Dateien

Gantner_F_Hepatology_1995.pdf
Gantner_F_Hepatology_1995.pdfGröße: 902.47 KBDownloads: 1329

Datum

1995

Autor:innen

Lohse, Ansgar Wilhelm
Germann, Paul Georg
Tiegs, Gisa

Herausgeber:innen

Kontakt

ISSN der Zeitschrift

Electronic ISSN

ISBN

Bibliografische Daten

Verlag

Schriftenreihe

Auflagebezeichnung

ArXiv-ID

Internationale Patentnummer

Angaben zur Forschungsförderung

Projekt

Open Access-Veröffentlichung
Open Access Green
Core Facility der Universität Konstanz

Gesperrt bis

Titel in einer weiteren Sprache

Publikationstyp
Zeitschriftenartikel
Publikationsstatus
Published

Erschienen in

Hepatology - Journal of American Association for the Study of the Liver Diseases. 1995, 21(1), pp. 190-198. ISSN 0270-9139. Available under: doi: 10.1016/0270-9139(95)90428-X

Zusammenfassung

Concanavalin A activates T lymphocytes in vitro and causes T-cell-dependent hepatic injury in mice. T lymphocytes were previously identified as effector cells of concanavalin A-induced liver injury. Here we report that hepatic injury is characterized by apoptotic cell death. On concanavalin A challenge, the cytokines tumor necrosis factor-α (TNFα), interleukin-2, granulocyte macrophage-colony stimulating factor, and interferon-γ were detectable in the circulation of the mice. Pretreatment of mice with anti-mouse TNF-α antiserum protected them from concanavalin A-induced liver injury. Nude mice failed to release TNF-α or interleukin-2 after concanavalin A challenge and were protected from liver injury. Lymph node cell transfer from responder mice to resistant nude mice resulted in susceptibility of the latter towards concanavalin A, i.e., to induction of cytokine release and hepatotoxicity. These experiments suggest that immunocompetent T cells play a pivotal role in concanavalin A-stimulated TNF-α release in vivo. After intravenous administration of fluorescein isothiocyanate-labeled concanavalin A to mice, the most fluorescence was found within the liver. In vitro, concanavalin A stimulation of separate cultures of mouse lymph node cells or nonparenchymal liver cells induced the release of minute amounts of TNF, whereas stimulation of cocultures of these cells resulted in production of substantial amounts of TNF-α. These findings may explain the hepatotropic effect of concanavalin A. In conclusion, T-cell-dependent concanavalin A-induced apoptotic liver injury in mice is related to immunological and cytokinemediated disorders and possibly to autoreactive hepatic processes.

Zusammenfassung in einer weiteren Sprache

Fachgebiet (DDC)
570 Biowissenschaften, Biologie

Schlagwörter

Konferenz

Rezension
undefined / . - undefined, undefined

Forschungsvorhaben

Organisationseinheiten

Zeitschriftenheft

Zugehörige Datensätze in KOPS

Zitieren

ISO 690GANTNER, Florian, Marcel LEIST, Ansgar Wilhelm LOHSE, Paul Georg GERMANN, Gisa TIEGS, 1995. Concanavalin A - induced T-cell - mediated hepatic injury in mice : The role of tumor necrosis factor. In: Hepatology - Journal of American Association for the Study of the Liver Diseases. 1995, 21(1), pp. 190-198. ISSN 0270-9139. Available under: doi: 10.1016/0270-9139(95)90428-X
BibTex
@article{Gantner1995Conca-8281,
  year={1995},
  doi={10.1016/0270-9139(95)90428-X},
  title={Concanavalin A - induced T-cell - mediated hepatic injury in mice : The role of tumor necrosis factor},
  number={1},
  volume={21},
  issn={0270-9139},
  journal={Hepatology - Journal of American Association for the Study of the Liver Diseases},
  pages={190--198},
  author={Gantner, Florian and Leist, Marcel and Lohse, Ansgar Wilhelm and Germann, Paul Georg and Tiegs, Gisa}
}
RDF
<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/8281">
    <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/8281"/>
    <dc:language>eng</dc:language>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:contributor>Tiegs, Gisa</dc:contributor>
    <dcterms:title>Concanavalin A - induced T-cell - mediated hepatic injury in mice : The role of tumor necrosis factor</dcterms:title>
    <dc:creator>Leist, Marcel</dc:creator>
    <dc:creator>Gantner, Florian</dc:creator>
    <dc:creator>Tiegs, Gisa</dc:creator>
    <dcterms:bibliographicCitation>First publ. in: Hepatology - Journal of American Association for the Study of the Liver Diseases 21 (1995), 1, pp. 190-198</dcterms:bibliographicCitation>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dc:creator>Germann, Paul Georg</dc:creator>
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/8281/1/Gantner_F_Hepatology_1995.pdf"/>
    <dc:contributor>Gantner, Florian</dc:contributor>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:42:23Z</dcterms:available>
    <dc:contributor>Lohse, Ansgar Wilhelm</dc:contributor>
    <dc:creator>Lohse, Ansgar Wilhelm</dc:creator>
    <dcterms:issued>1995</dcterms:issued>
    <dc:contributor>Germann, Paul Georg</dc:contributor>
    <dc:contributor>Leist, Marcel</dc:contributor>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:42:23Z</dc:date>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:rights>terms-of-use</dc:rights>
    <dcterms:abstract xml:lang="eng">Concanavalin A activates T lymphocytes in vitro and causes T-cell-dependent hepatic injury in mice. T lymphocytes were previously identified as effector cells of concanavalin A-induced liver injury. Here we report that hepatic injury is characterized by apoptotic cell death. On concanavalin A challenge, the cytokines tumor necrosis factor-α (TNFα), interleukin-2, granulocyte macrophage-colony stimulating factor, and interferon-γ were detectable in the circulation of the mice. Pretreatment of mice with anti-mouse TNF-α antiserum protected them from concanavalin A-induced liver injury. Nude mice failed to release TNF-α or interleukin-2 after concanavalin A challenge and were protected from liver injury. Lymph node cell transfer from responder mice to resistant nude mice resulted in susceptibility of the latter towards concanavalin A, i.e., to induction of cytokine release and hepatotoxicity. These experiments suggest that immunocompetent T cells play a pivotal role in concanavalin A-stimulated TNF-α release in vivo. After intravenous administration of fluorescein isothiocyanate-labeled concanavalin A to mice, the most fluorescence was found within the liver. In vitro, concanavalin A stimulation of separate cultures of mouse lymph node cells or nonparenchymal liver cells induced the release of minute amounts of TNF, whereas stimulation of cocultures of these cells resulted in production of substantial amounts of TNF-α. These findings may explain the hepatotropic effect of concanavalin A. In conclusion, T-cell-dependent concanavalin A-induced apoptotic liver injury in mice is related to immunological and cytokinemediated disorders and possibly to autoreactive hepatic processes.</dcterms:abstract>
    <dc:format>application/pdf</dc:format>
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/8281/1/Gantner_F_Hepatology_1995.pdf"/>
    <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/>
  </rdf:Description>
</rdf:RDF>

Interner Vermerk

xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter

Kontakt
URL der Originalveröffentl.

Prüfdatum der URL

Prüfungsdatum der Dissertation

Finanzierungsart

Kommentar zur Publikation

Allianzlizenz
Corresponding Authors der Uni Konstanz vorhanden
Internationale Co-Autor:innen
Universitätsbibliographie
Nein
Begutachtet
Diese Publikation teilen