Structure and abnormalities in cones of the Wollemi pine (Wollemia nobilis)

Abstract

Reproductive structures of both genders of Wollemia nobilis were investigated, including both wild-type and teratological cones. Typically, both pollen cones and seed cones in this species are terminal on first order branches. At maturity, wild-type pollen cones are pendulous and cylindrical; wild-type seed cones are broad and ellipsoidal in shape. The teratological structures consisted of a basal region that resembled a typical fertile seed cone, and an apical proliferation that terminated in a well-developed pollen cone, resulting in a ‘bisexual’ unit. The proximal seed cone and the distal pollen cone were separated by a sterile region that represents an elongation of the cone axis. Of a total of 14 anomalous bisexual units investigated, all had the same bauplan. Such an arrangement of basal ovulate and distal staminate reproductive structures in a teratological conifer cone has previously not been reported for Wollemia. This topology is 'inside-out' with respect to most other reported anomalous bisexual conifer cones, which possess proximal staminate and distal ovulate structures. We discuss these spontaneous abnormalities in the broader context of understanding the homologies of seed-plant reproductive structures. The patterning of conifer cones is apparently highly labile, perhaps related to the extended cone axis and relatively long developmental duration.