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Crystallization of human low density lipoprotein (LDL), a large lipid-protein complex : collection of X-ray data at very low resolution

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35_Ritter_JCrystGrowth196_1999_344.pdf
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1999

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Ritter, Stephan
Frey, Ingrid
Berg, Aloys
Keul, Joseph
Baumstark, Manfred W.

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Journal of Crystal Growth. 1999, 196(2-4), pp. 344-349. ISSN 0022-0248. Available under: doi: 10.1016/S0022-0248(98)00846-X

Zusammenfassung

Human LDL subfractions LDL-2 (d=1.031-1.034 g/ml) and LDL-5 (d=1.040-1.044 g/ml) were crystallized in different crystal forms using polyethylene glycol as a precipitant. Both fractions were from one donor. Crystals of LDL-5 were yellow, hexagonal, and showed no dichroism. Of LDL-2 two dichroitic crystal forms were obtained. One had a rod-like shape with deep notches at both ends (form A), the other was a more compact form with plain surfaces (form B). To be able to measure low order reflections down to 300 Å a special experimental setup was developed. One single crystal was used to obtain a complete native data set of LDL-2 (form A) with an overall internal R-factor of 4.5% for reflections from 100 to 28 Å. Data were collected under cryogenic conditions using synchrotron radiation. The space group is most probably C2 with unit cell dimensions of a=183 Å, b=421 Å, c=385 Å, α=γ=90°, β≈90°. Further optimization of the crystallization conditions and the search for heavy metal derivatives are in progress.

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Fachgebiet (DDC)
570 Biowissenschaften, Biologie

Schlagwörter

Low density lipoprotein, Atherosclerosis, Crystal, Data collection

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ISO 690RITTER, Stephan, Kay DIEDERICHS, Ingrid FREY, Aloys BERG, Joseph KEUL, Manfred W. BAUMSTARK, 1999. Crystallization of human low density lipoprotein (LDL), a large lipid-protein complex : collection of X-ray data at very low resolution. In: Journal of Crystal Growth. 1999, 196(2-4), pp. 344-349. ISSN 0022-0248. Available under: doi: 10.1016/S0022-0248(98)00846-X
BibTex
@article{Ritter1999Cryst-8616,
  year={1999},
  doi={10.1016/S0022-0248(98)00846-X},
  title={Crystallization of human low density lipoprotein (LDL), a large lipid-protein complex : collection of X-ray data at very low resolution},
  number={2-4},
  volume={196},
  issn={0022-0248},
  journal={Journal of Crystal Growth},
  pages={344--349},
  author={Ritter, Stephan and Diederichs, Kay and Frey, Ingrid and Berg, Aloys and Keul, Joseph and Baumstark, Manfred W.}
}
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