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Mitochondria are devoid of poly(ADP-ribose)polymerase-1, but harbor its product oligo(ADP-ribose)

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2021

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Köritzer, Julia
Blenn, Christian

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Journal of Cellular Biochemistry. Wiley. 2021, 122(5), pp. 507-523. ISSN 0730-2312. eISSN 1547-9366. Available under: doi: 10.1002/jcb.29887

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There are conflicting data about localization of poly(ADP-ribose)polymerase-1 and its product poly(ADP-ribose) in mitochondria. To finally clarify the discussion, we investigated with biochemical and cell biological methods the potential presence of poly(ADP-ribose) polymerase-1 in these organelles. Our data show that endogenous and overexpressed poly(ADP-ribose)polymerase 1 is only localized to the nucleus with a clear exclusion of cytosolic compartments. In addition, highly purified mitochondria devoid of nuclear contaminations do not contain poly(ADP-ribose)polymerase-1. Although no poly(ADP-ribose)polymerase-1 enzyme is detectable in mitochondria, a shorter variant of its product poly(ADP-ribose) is present, associated specifically with a small subset of mitochondrial proteins as revealed by immunoprecipitation and protein fingerprint analysis. These proteins are located at key-points of the Krebs-cycle, are chaperones involved in mitochondrial functionality and quality-control, and are RNA-binding proteins important for transcript stability, respectively. Of note, despite the fact that especially poly(ADP-ribose)polymerase-1 is its own major target for modification, we could not detect this enzyme by mass spectrometry in these organelles. These data suggests a new way of targeted nuclear-mitochondrial signaling, mediated by nuclear poly(ADP-ribosyl)ation dependent on poly(ADP-ribose)polymerase-1.

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570 Biowissenschaften, Biologie

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ISO 690KÖRITZER, Julia, Christian BLENN, Alexander BÜRKLE, Sascha BENEKE, 2021. Mitochondria are devoid of poly(ADP-ribose)polymerase-1, but harbor its product oligo(ADP-ribose). In: Journal of Cellular Biochemistry. Wiley. 2021, 122(5), pp. 507-523. ISSN 0730-2312. eISSN 1547-9366. Available under: doi: 10.1002/jcb.29887
BibTex
@article{Koritzer2021-05Mitoc-52275,
  year={2021},
  doi={10.1002/jcb.29887},
  title={Mitochondria are devoid of poly(ADP-ribose)polymerase-1, but harbor its product oligo(ADP-ribose)},
  number={5},
  volume={122},
  issn={0730-2312},
  journal={Journal of Cellular Biochemistry},
  pages={507--523},
  author={Köritzer, Julia and Blenn, Christian and Bürkle, Alexander and Beneke, Sascha}
}
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