Publikation:

Acetone utilization by sulfate-reducing bacteria : draft genome sequence of Desulfococcus biacutus and a proteomic survey of acetone-inducible proteins

Vorschaubild

Dateien

Acosta_286630.pdf
Acosta_286630.pdfGröße: 728.97 KBDownloads: 278

Datum

2014

Autor:innen

Gutiérrez Acosta, Olga B.

Herausgeber:innen

Kontakt

ISSN der Zeitschrift

Electronic ISSN

ISBN

Bibliografische Daten

Verlag

Schriftenreihe

Auflagebezeichnung

URI (zitierfähiger Link)
http://nbn-resolving.de/urn:nbn:de:bsz:352-286630
DOI (zitierfähiger Link)
https://doi.org/10.1186/1471-2164-15-584
ArXiv-ID

Internationale Patentnummer

Link zur Lizenz
Urheberrechtlich geschützt

Angaben zur Forschungsförderung

Projekt

Open Access-Veröffentlichung
Open Access Gold

Sammlungen

Biologie: Publikationen
Core Facility der Universität Konstanz

Gesperrt bis

Titel in einer weiteren Sprache

Publikationstyp
Zeitschriftenartikel
Publikationsstatus
Published

Erschienen in

BMC Genomics. 2014, 15(1), 584. eISSN 1471-2164. Available under: doi: 10.1186/1471-2164-15-584

Zusammenfassung

Background
The sulfate-reducing bacterium Desulfococcus biacutus is able to utilize acetone for growth by an inducible degradation pathway that involves a novel activation reaction for acetone with CO as a co-substrate. The mechanism, enzyme(s) and gene(s) involved in this acetone activation reaction are of great interest because they represent a novel and yet undefined type of activation reaction under strictly anoxic conditions.

Results
In this study, a draft genome sequence of D. biacutus was established. Sequencing, assembly and annotation resulted in 159 contigs with 5,242,029 base pairs and 4773 predicted genes; 4708 were predicted protein-encoding genes, and 3520 of these had a functional prediction. Proteins and genes were identified that are specifically induced during growth with acetone. A thiamine diphosphate-requiring enzyme appeared to be highly induced during growth with acetone and is probably involved in the activation reaction. Moreover, a coenzyme B12- dependent enzyme and proteins that are involved in redox reactions were also induced during growth with acetone.

Conclusions
We present for the first time the genome of a sulfate reducer that is able to grow with acetone. The genome information of this organism represents an important tool for the elucidation of a novel reaction mechanism that is employed by a sulfate reducer in acetone activation.

Zusammenfassung in einer weiteren Sprache

Fachgebiet (DDC)
570 Biowissenschaften, Biologie

Schlagwörter

Konferenz

Rezension
undefined / . - undefined, undefined

Forschungsvorhaben

Organisationseinheiten

Zeitschriftenheft

Zugehörige Datensätze in KOPS

Zitieren

ISO 690GUTIÉRREZ ACOSTA, Olga B., David SCHLEHECK, Bernhard SCHINK, 2014. Acetone utilization by sulfate-reducing bacteria : draft genome sequence of Desulfococcus biacutus and a proteomic survey of acetone-inducible proteins. In: BMC Genomics. 2014, 15(1), 584. eISSN 1471-2164. Available under: doi: 10.1186/1471-2164-15-584
BibTex
@article{GutierrezAcosta2014Aceto-28663,
  year={2014},
  doi={10.1186/1471-2164-15-584},
  title={Acetone utilization by sulfate-reducing bacteria : draft genome sequence of Desulfococcus biacutus and a proteomic survey of acetone-inducible proteins},
  number={1},
  volume={15},
  journal={BMC Genomics},
  author={Gutiérrez Acosta, Olga B. and Schleheck, David and Schink, Bernhard},
  note={Article Number: 584}
}
RDF
<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28663">
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2014-08-05T08:47:44Z</dcterms:available>
    <dc:creator>Gutiérrez Acosta, Olga B.</dc:creator>
    <dc:language>eng</dc:language>
    <dc:creator>Schink, Bernhard</dc:creator>
    <dc:rights>terms-of-use</dc:rights>
    <dc:contributor>Schleheck, David</dc:contributor>
    <dcterms:issued>2014</dcterms:issued>
    <dc:creator>Schleheck, David</dc:creator>
    <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/>
    <dcterms:bibliographicCitation>BMC Genomics ; 15 (2014). - 584</dcterms:bibliographicCitation>
    <dcterms:abstract xml:lang="eng">Background&lt;br /&gt;The sulfate-reducing bacterium Desulfococcus biacutus is able to utilize acetone for growth by an inducible degradation pathway that involves a novel activation reaction for acetone with CO as a co-substrate. The mechanism, enzyme(s) and gene(s) involved in this acetone activation reaction are of great interest because they represent a novel and yet undefined type of activation reaction under strictly anoxic conditions.&lt;br /&gt;&lt;br /&gt;Results&lt;br /&gt;In this study, a draft genome sequence of D. biacutus was established. Sequencing, assembly and annotation resulted in 159 contigs with 5,242,029 base pairs and 4773 predicted genes; 4708 were predicted protein-encoding genes, and 3520 of these had a functional prediction. Proteins and genes were identified that are specifically induced during growth with acetone. A thiamine diphosphate-requiring enzyme appeared to be highly induced during growth with acetone and is probably involved in the activation reaction. Moreover, a coenzyme B&lt;sub&gt;12&lt;/sub&gt;- dependent enzyme and proteins that are involved in redox reactions were also induced during growth with acetone.&lt;br /&gt;&lt;br /&gt;Conclusions&lt;br /&gt;We present for the first time the genome of a sulfate reducer that is able to grow with acetone. The genome information of this organism represents an important tool for the elucidation of a novel reaction mechanism that is employed by a sulfate reducer in acetone activation.</dcterms:abstract>
    <dc:contributor>Schink, Bernhard</dc:contributor>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2014-08-05T08:47:44Z</dc:date>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/28663"/>
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/28663/1/Acosta_286630.pdf"/>
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/28663/1/Acosta_286630.pdf"/>
    <dc:contributor>Gutiérrez Acosta, Olga B.</dc:contributor>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dcterms:title>Acetone utilization by sulfate-reducing bacteria : draft genome sequence of Desulfococcus biacutus and a proteomic survey of acetone-inducible proteins</dcterms:title>
  </rdf:Description>
</rdf:RDF>

Interner Vermerk

xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter

Kontakt
URL der Originalveröffentl.

Prüfdatum der URL

Prüfungsdatum der Dissertation

Finanzierungsart

Kommentar zur Publikation

Allianzlizenz
Corresponding Authors der Uni Konstanz vorhanden
Internationale Co-Autor:innen
Universitätsbibliographie
Ja
Begutachtet
Diese Publikation teilen