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Hydrophobic and hydrophilic radio-iodination, crosslinking, and differential extraction of cell surface proteins in Paramecium tetraurelia cells

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Hydrophobic_and_hydrophilic_.pdf
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1999

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Flötenmeyer, Matthias
Momayezi, Massoud

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Journal of Membrane Biology. 1999, 172(1), pp. 77-88. ISSN 0022-2631. eISSN 1432-1424. Available under: doi: 10.1007/s002329900585

Zusammenfassung

We combined widely different biochemical methods to analyze proteins of the cell surface of P. tetraurelia since so far one can isolate only a subfraction of cell membrane vesicles enriched in the GPI-anchored surface antigens ( immoblization or i-AGs ). We also found that i-AGs may undergo partial degradation by endogenous proteases. Genuine intrinsic membrane proteins were recognized particularly with lipophilic 5-[125I]-iodonaphthalene-1-azide (INA) labeling which reportedly sees integral proteins and cytoplasmic cell membrane-associated proteins. With INA (+DTT), bands of ≤ 55 kDa were similar as with hydrophilic iodogen (+DTT), but instead of large size bands including i-AGs, a group of 122, 104 and 94 kDa appeared. Several bands of the non i-AG type are compatible with integral (possibly oligomeric) or associated proteins of the cell membrane of established molecular identity, as we discuss. In summary, we can discriminate between i-AGs and some functionally important minor cell membrane components. Our methodical approach might be relevant also for an analysis of some related protozoan parasites.

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570 Biowissenschaften, Biologie

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Cell membrane, Ciliates, Crosslinking, Iodination, Paramecium, Proteins

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ISO 690FLÖTENMEYER, Matthias, Massoud MOMAYEZI, Helmut PLATTNER, 1999. Hydrophobic and hydrophilic radio-iodination, crosslinking, and differential extraction of cell surface proteins in Paramecium tetraurelia cells. In: Journal of Membrane Biology. 1999, 172(1), pp. 77-88. ISSN 0022-2631. eISSN 1432-1424. Available under: doi: 10.1007/s002329900585
BibTex
@article{Flotenmeyer1999Hydro-7487,
  year={1999},
  doi={10.1007/s002329900585},
  title={Hydrophobic and hydrophilic radio-iodination, crosslinking, and differential extraction of cell surface proteins in Paramecium tetraurelia cells},
  number={1},
  volume={172},
  issn={0022-2631},
  journal={Journal of Membrane Biology},
  pages={77--88},
  author={Flötenmeyer, Matthias and Momayezi, Massoud and Plattner, Helmut}
}
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